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Updated: Jun 7, 2026

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
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纯化,结晶和结构确定mpox病毒mRNA帽N7甲基转移酶复合物的协议

Qingqing Guo1, Anke Chen1, Jin Ding2

  • 1School of Life Sciences and Pudong Hospital, Fudan University, Shanghai Engineering Research Center of Industrial Microorganisms, Shanghai 200438, China.

STAR protocols
|May 31, 2025
PubMed
概括

这项研究详细介绍了净化和结晶mpox病毒 (MPXV) mRNA帽N7甲基转移酶 (RNMT) 复合物的协议. 这一框架有助于对病毒酶进行结构分析,用于免疫逃避研究.

关键词:
分子生物学分子生物学蛋白质的表达和净化过程结构生物学 结构生物学在X射线晶体学研究中,

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相关概念视频

Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...