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在人类黑色素瘤细胞中分析miRNA的协议

Evelyn Lattmann1, Tessa Guggiari1, Patrick Turko1

  • 1Department of Dermatology, University of Zurich, University Hospital Zurich, 8952 Schlieren, Switzerland.

STAR protocols
|May 31, 2025
PubMed
概括

本研究提出了分析人类黑色素瘤细胞中微RNA (miRNA) 的协议,重点关注表达和同定位. 该方法有助于了解黑色素瘤的可塑性,转移和治疗耐药性.

关键词:
癌症 癌症 癌症 癌症细胞生物学 细胞生物学细胞培养培养的细胞培养.基于细胞的测试在现场混合化.显微镜的使用方法

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MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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