饮食中的白醇可以缓解养子系统下的子的肝脏和肠道损伤
Yong Tian1, Xiangshan Liu2, Li Chen1
1State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China; Zhejiang Provincial Key Laboratory of Livestock and Poultry Biotech Breeding, Hangzhou, 310021, China.
复星 (RES) 补充剂可以缓解养子的子的肝脏和肠道损伤. 它增强抗氧化能力,减少炎症,细胞内网膜应激和亡,同时激活PI3K/AKT通路.
科学领域:
- 动物科学动物科学
- 营养生物化学 营养生物化学
- 禽类的健康 禽类的健康
背景情况:
- 子养在子中的做法可以诱导氧化应激和炎症.
- 复星 (Resveratrol,简称RES) 是一种天然的多,具有抗氧化和抗炎性质.
- 了解RES对农业引起的压力的保护机制对于子的福利至关重要.
研究的目的:
- 为了评估饮食中食 (RES) 对子养引起的肝脏和十二指肠健康问题的保护作用.
- 调查氧化还原失衡,炎症,内分泌网膜 (ER) 应激,细胞亡以及PI3K/AKT和MAPK/ERK通路在RES的保护作用中的参与.
主要方法:
- 在养条件下,120只雌被分配到控制或补充RES (500毫克/公斤) 的饮食中.
- 血液,肝脏和十二指肠样本在10天内被采集用于组织病理学,生物化学和分子分析.
- 量化实时PCR和西式斑点测试用于评估与压力和信号通路相关的基因和蛋白质表达.
主要成果:
- 补充RES可以减少组织病理损伤,改善肝功能标志物 (ALT,AST).
- RES增强了抗氧化剂状态 (增加了CAT,GSH-Px,SOD,T-AOC;减少了MDA) 和抑制了炎症 (减少了IL-1β,IL-6;增加了IL-4).
- RES干预使炎症 (iNOS,COX2),ER压力 (GRP78) 和亡 (Bax,Bcl2) 标记的mRNA水平正常化,并促进了AKT酸化,同时减少了分裂的caspase-3.
结论:
- ресвератрол有效地减轻子养引起的肝脏和肠道损伤.
- RES通过增强抗氧化剂防御,抑制炎症,ER压力和亡来发挥其保护作用.
- 激活PI3K/AKT信号通路是RES在养子中的有益作用的关键机制.
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