由共存的纳米塑料诱导的三甲与catalase的增强结合
Qi Lou1, Rongguang Shi2, Rui Guan1
1School of Environment and Geography, Qingdao University, Qingdao 266071, China.
概括
三碳素 (TCC) 和纳米塑料一起增加了比单独的TCC更多的催化酶 (CAT) 的活性. 这是因为纳米塑料会导致CAT的结构变化,增强TCC的结合,导致更大的氧化应激.
科学领域:
- 环境毒理学环境毒理学
- 生物化学 生化学
- 纳米技术 纳米技术
背景情况:
- 三碳 (TCC) 是一种环境污染物,影响着酶 (CAT) 活性,并引起氧化应激.
- 纳米塑料可以与蛋白质结合,改变它们的功能,但在分子层面上,与TCC的联合毒性尚不清楚.
研究的目的:
- 研究聚乙烯纳米塑料 (PSNPs) 对结合酶 (CAT) 的三碳素 (TCC) 的影响.
- 阐明TCC和PSNP对CAT活动的联合毒性背后的分子机制.
主要方法:
- 光谱学 (光,紫外线可见,圆形二重化)
- 酶活性检测试验测定了酶活性.
- 动态光散射分析
- 分子对接是分子对接.
主要成果:
- 单独使用TCC时,CAT活动增加到131%,使用TCC和PSNP时增加到138%.
- 在PSNPs的表面上,CAT形成了一个蛋白质冠状.
- PSNP加剧了TCC诱导的CAT结构变化,包括脊柱松和降低α-螺旋体含量,增强TCC结合.
结论:
- 与单独的TCC相比,对PSNP和TCC的联合暴露会导致CAT活动增加.
- 蛋白质冠状形成和PSNP对CAT的增强形状变化有助于活动升高.
- 这些发现有助于更好地理解与TCC和PSNP同时存在的氧化损伤.
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