在透镜上皮细胞中,LIPUS对表皮-介质细胞过渡的抑制作用
Junfen Li1, Yiqing Zhou1, Sicheng He1
1State Key Laboratory of Ultrasound in Medicine and Engineering, Chongqing Medical University, Chongqing, China; Chongqing Key Laboratory of Biomedical Engineering, Chongqing Medical University, Chongqing, China.
Experimental eye research
|May 31, 2025
概括
低强度脉冲超声波 (LIPUS) 通过恢复自和重塑细胞骨架来抑制镜片细胞中高葡萄糖诱导的上皮层-介质细胞过渡 (EMT). 这项研究揭示了LIPUS作为EMT相关眼睛疾病的潜在治疗方法.
科学领域:
- 眼科医生 眼科 眼科
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
背景情况:
- 表皮-介质细胞过渡 (EMT) 与各种眼部疾病有关.
- 高葡萄糖 (HG) 是已知的诱导器的EMT在镜头上皮细胞.
- 了解调节高温诱导EMT的机制对于开发治疗策略至关重要.
研究的目的:
- 研究低强度脉冲超声波 (LIPUS) 在SRA01/04镜片上皮细胞中减轻高压诱导的EMT的治疗潜力.
- 阐明自和细胞骨架重塑在LIPUS介导的EMT抑制中的作用.
主要方法:
- 在SRA01/04细胞中使用高葡萄糖 (HG) 诱导EMT.
- 细胞计数工具-8试验,流细胞计量,光显微镜和伤口愈合试验被用来评估细胞活力,形态和迁移.
- 免疫光 (IF) 染色,西斑 (WB),法洛伊丁染色和传输电子显微镜被用来分析蛋白质水平,自标记物和细胞骨结构.
主要成果:
- LIPUS治疗逆转了HG诱导的形态变化,减少了细胞迁移,并增加了E-cadherin水平.
- 利普斯上调的自标志物 (LC3B/LC3A) 和下调的EMT标志物 (α-SMA) 和自基质 (SQSTM1/P62).
- 用LIPUS治疗调节的EMT标记物和细胞迁移抑制自或细胞骨动力学,表明它们参与LIPUS的机制.
结论:
- 利普斯在透镜上皮细胞中有效抑制高葡萄糖诱导的EMT.
- 利普斯对EMT的抑制作用是通过恢复自和随后的细胞骨重塑来实现的.
- 在EMT相关的眼部病理方面,LIPUS显示出作为一种非侵入性治疗方式的潜力.
相关概念视频
01:24Cells of the Epidermis
The epidermis is made of four or five layers of epithelial cells, depending on its location in the body. From deep to superficial, these layers are the stratum basale, stratum spinosum, stratum granulosum, stratum lucidum, and stratum corneum.
The cells in all these layers except the stratum basale are called keratinocytes, a type of cell that manufactures and stores the protein keratin. The keratinocytes in the stratum corneum are dead and regularly slough away, being replaced by cells from...
The cells in all these layers except the stratum basale are called keratinocytes, a type of cell that manufactures and stores the protein keratin. The keratinocytes in the stratum corneum are dead and regularly slough away, being replaced by cells from...
01:11Papillary Dermis
Dermis
The dermis might be considered the "core" of the integumentary system, as distinct from the epidermis and hypodermis. It contains blood and lymph vessels, nerves, and other structures, such as hair follicles and sweat glands. The dermis is made of two layers of connective tissue that comprise an interconnected mesh of elastin and collagenous fibers, produced by fibroblasts.
Papillary Layer
The papillary layer is made of loose, areolar connective tissue, which means the collagen and...
The dermis might be considered the "core" of the integumentary system, as distinct from the epidermis and hypodermis. It contains blood and lymph vessels, nerves, and other structures, such as hair follicles and sweat glands. The dermis is made of two layers of connective tissue that comprise an interconnected mesh of elastin and collagenous fibers, produced by fibroblasts.
Papillary Layer
The papillary layer is made of loose, areolar connective tissue, which means the collagen and...