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人类细胞巨型病毒蛋白US18促进NLRP3炎症酶激活

Yu Zhang¹, Ya-Li Lei², Peng Ren²

¹College of Life Sciences, Liaoning University, Shenyang, China; Department of Infectious Diseases, Frontier Science Center for Immunology and Metabolism, Medical Research Institute, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, China.

Biochemical and biophysical research communications

|June 1, 2025

概括

人类细胞巨型病毒 (HCMV) 蛋白US18激活NLRP3炎症体,驱动炎症性细胞因子释放和细胞死亡. 这一发现澄清了HCMV感染如何在单细胞中引发显著的炎症反应.

科学领域:

免疫学免疫学免疫学
病毒学病毒学
细胞生物学细胞生物学

背景情况:

人类细胞巨型病毒 (HCMV) 感染单细胞会通过炎症酶激活,细胞因子释放 (IL-1β,IL-18) 和火致死引发炎症.
目前尚不完全了解HCMV诱导这些炎症反应的确切机制.

研究的目的:

阐明特定HCMV蛋白在单细胞感染期间启动炎症级联中的作用.
确定负责NLRP3炎症酶激活和随后的炎症结果的病毒因素.

主要方法:

在单细胞感染模型中研究了HCMV蛋白US18的功能.
使用共免疫沉试验来检查US18和炎症组分 (NEK7,NLRP3,亲IL-1β) 之间的相互作用.
评估了US18敲击或表达后的炎症酶激活,细胞因子释放和热.

主要成果:

鉴定出HCMV蛋白US18是NLRP3炎症酶激活的关键促进体.
US18直接与NLRP3炎症酶组件结合,促进NLRP3的寡合化和斑点形成.
击败US18显著抑制了HCMV诱导的NLRP3炎症酶激活,IL-1β释放和热.

结论:

在HCMV感染期间,HCMV蛋白US18在积极调节NLRP3炎症酶激活方面发挥着至关重要的作用.
US18是炎症反应的重要驱动因素,包括IL-1β释放和热,在HCMV感染单细胞后.
针对US18可能提供一种策略来调节HCMV诱导的炎症.

关键词:

人类细胞巨型病毒病毒.在NLRP3炎症酶体中,NLRP3炎症热灭菌的发生.美国18美元

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