在异常性肺纤维化中使用青飞蒸:针对肺部的成分和PI3K-AKT调制
Yongqi Liu1, Luyao Song2, Shiyi Chen2
1School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China; Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Zhongshan, 528400, China.
青飞脱剂 (QFY) 通过影响肺细胞和PI3K-AKT通路,显示出治疗异常性肺纤维化 (IPF) 的潜力. 在这种传统中医药配方中,Oroxylin A和Baicalin等关键化合物被认为是有益的.
科学领域:
- 传统中国医药 传统中国医药
- 肺纤维化研究研究
- 药理学 药理学是指药理学的学科.
背景情况:
- 青飞 (Qingfeiyin) (QFY) 是一种用于肺部疾病的传统中医配方.
- 它在治疗异常性肺纤维化 (IPF) 的疗效在很大程度上仍未得到研究.
研究的目的:
- 评估QFY对IPF的治疗效果.
- 确定QFY的生物活性成分并阐明它们的作用机制.
主要方法:
- 建立了白胺诱导的肺纤维化小鼠模型,以评估QFY的抗IPF作用.
- 利用HPLC-MS识别生物活性成分,以及用于功能分析的体外细胞模型 (EMT,FMT,巨分化).
- 综合网络药理学和临床数据 (GSE110147) 预测机制,通过体外实验,分子对接和模拟进行验证.
主要成果:
- QFY证明了IPF病理表型的剂量依赖性改善.
- 鉴定了Oroxylin A (OA),Geniposide (GDS),Baicalin (BCL) 和Genipin-1-gentiobioside (GG) 与肺丰富;OA和BCL显著改善了EMT,FMT和巨细胞两极分化.
- 网络药理学和生物信息学将OA/GDS/BCL/GG与PI3K-AKT通路联系起来,通过对细胞迁移和细胞亡的影响得到证实. 分子对接显示了BCL和OA与PI3K子单元的强有力的结合.
结论:
- QFY显示了IPF治疗的治疗潜力.
- QFY中的生物活性成分可能通过调节肺组织中的PI3K信号通路来发挥作用.
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