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乳清蛋白水解剂通过激活米托法基来缓解了乙氨基诱导的肝细胞热

S Zhang¹, J Guo¹, M Liu¹

¹College of Food Science and Engineering, Jilin University, Changchun, Jilin 130062, China.

Journal of dairy science

|June 1, 2025

概括

乳清蛋白水解剂 (WPH) 通过减少热死来减轻乙氨基诱导的肝损伤. WPH激活了髓,减少了活性氧物种和NLRP3炎症酶激活,保护肝细胞.

科学领域:

生物化学生化学
细胞生物学细胞生物学
毒理学毒理学毒理学

背景情况:

过量服用乙氨基 (APAP) 会导致肝损伤,部分原因是通过激素灭亡,这是一个被编程细胞死亡的途径.
乳清蛋白水解剂 (WPH) 在预防和治疗APAP诱导的肝损伤方面具有潜力.
对于WPH对APAP诱导的热死作用的精确机制需要进一步阐明.

研究的目的:

研究WPH对APAP诱导的肝细胞损伤和热的保护机制.
探索氧化应激,溶酶体完整性和线粒细胞衰减在WPH治疗效果中的作用.

主要方法:

在APAP暴露和WPH治疗后,评估了肝细胞损伤和热的标志物.
测量了反应性氧物种 (ROS),溶酶体甲素B (CTSB) 和与热相关的蛋白质 (NLRP3,GSDMD-N,CASP1) 的水平.
评估了与线粒相关的蛋白质 (PRKN,PINK1,LC3,P62) 和线粒激活,并使用线粒抑制剂进行验证.

主要成果:

WPH显著降低了APAP诱导的肝细胞损伤和IL-1β和IL-18的释放.
治疗WPH降低了热症标志物的表达 (NLRP3,GSDMD-N,切割的CASP1) 和细胞内ROS水平.
WPH 抑制了 CTSB 释放,调高了与髓相关的蛋白质 (PRKN,PINK1,LC3) 和调低了P62,表明髓激活.

结论:

通过抑制热致死,WPH可以防止APAP诱导的肝损伤.
WPH激活了线粒,导致ROS积累减少和NLRP3炎症酶激活.
乙氨基 (WPH) 是一种潜在的治疗策略,用于控制乙氨基引起的肝毒性.

关键词:

乙氨基的使用方法线粒细胞衰变 (mitophagy) 是一种神经衰变的过程.热致灭 (pyroptosis) 是一种致的过程.whey 蛋白质的水解酶

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