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在调节的免疫反应中发现和描述Vibrio parahaemolyticus AHPND响应的piRNAs

Waruntorn Luangtrakul¹, Phattarunda Jaree², Kunlaya Somboonwiwat¹

¹Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.

Fish & shellfish immunology

|June 1, 2025

概括

这项研究确定了 (Penaeus vannamei) 中的piRNA同类物,并揭示了它们在调节对Vibrio parahaemolyticus AHPND (VP_AHPND) 感染的免疫反应中的作用,为的抗菌免疫提供了新的见解.

科学领域:

* 分子生物学 * 分子生物学
* 免疫学免疫学
* * 海洋生物学海洋生物学

背景情况:

*Piwi相互作用RNAs (piRNAs) 是基因表达的关键调节者,主要以转子子控制而闻名.
*虽然piRNAs与的病毒防御有关,但它们在抗菌免疫中的功能尚不清楚.
* 这项研究重点关注Penaeus vannamei,这是一个商业上重要的鱼种类.

研究的目的:

* 在Vibrio parahaemolyticus AHPND (VP_AHPND) 感染期间识别和描述Penaeus vannamei血细胞中的piRNA.
* 调查在的抗菌免疫反应中发现的piRNAs的调节作用.
*阐明基底的分子机制piRNA介导免疫力对VP_AHPND.

主要方法:

*重新分析了来自VP_AHPND挑战的血细胞的小RNA测序数据.
*使用P. vannamei转录组数据库识别piRNA同源及其预测的基因.
*使用piRNA模仿和基因敲除进行实验验证,随后进行表达特征和感染检测.

主要成果:

*确定了150个piRNA同类物,其中6个在VP感染后显著失调.
*piRNA和基因表达之间的负相关性表明有调节作用.
* piR-pva-29948104的特征是,它准PvRNF26 (一种STING正义基因),其模仿抑制PvRNF26,激活下游免疫基因.

结论:

*PiRNA同类在VP感染期间在Penaeus vannamei中调节免疫基因表达方面发挥着重要作用.
*已识别的piRNAs,特别是piR-pva-29948104,参与调节STING-IKKβ-Relish免疫通路.
*这项研究为中介于piRNAs的抗菌防御机制提供了新的见解,突出了疾病控制的潜在目标.

关键词:

抗菌反应抗菌反应在PIWI-交互RNA (piRNA) 中.佩内乌斯 (Penaeus vannamei) 是一个水族群.这种病毒是Vibrio parahaemolyticus AHPND (VP(AHPND)) 的.

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