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Updated: Sep 19, 2025

Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques

Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques

Published on: November 2, 2018

细胞力动力学的量化,无标签的映射

Xinyu Zhou^1,2, Ryan Porter^1,3, Xiaoyan Zhou^1,3

¹Biodesign Center for Bioelectronics and Biosensors, Arizona State University, Tempe, AZ, 85287, USA.

Small (Weinheim an der Bergstrasse, Germany)

|June 1, 2025

概括

无标签的等离子细胞力显微镜实时绘制高分辨率的细胞力图. 这种技术揭示了心肌细胞动态和人口水平的细胞反应,推动了细胞力学研究.

科学领域:

生物物理学的生物物理.
细胞生物学细胞生物学
显微镜的使用方法

背景情况:

细胞力量对于生物过程至关重要.
测量细胞力现有的方法往往缺乏空间分辨率或需要标签.

研究的目的:

开发和演示一种无标签的等离子体细胞力显微镜技术.
为了量化细胞所施加的力量,具有衍射有限的空间分辨率和毫秒时间分辨率.

主要方法:

利用等离子散射成像来实时量化细胞基质相互作用动态.
开发了一种无标签显微镜方法,用于绘制细胞力图.

主要成果:

准确地绘制了细胞力量的空间和时间演变.
用毫秒分辨率测量心肌细胞力演变和加载率.
观察到异质细胞力变化,以响应尼古丁受体激活.

结论:

开发的无标签的等离子细胞力显微镜为研究细胞力学提供了多功能工具.
这种技术提供了高空间和时间分辨率的实时力映射.
证明了调查人口水平细胞反应和药物效应的潜力.

关键词:

生物传感器生物传感器细胞的力量是细胞的力量.没有标签的 label-free.塑散射显微镜的使用方法

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Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

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Integrative Toolkit to Analyze Cellular Signals: Forces, Motion, Morphology, and Fluorescence

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相关概念视频

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...