相关实验视频

Updated: Sep 19, 2025

Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis

Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis

Published on: June 14, 2019

在病变严重程度和疫苗接种之间,配对的宫细胞学样本和活检之间的HPV基因型化协议

Shimin Chen¹, Qiaoyun Du^1,2, Jian Yin^1,3

¹Department of Cancer Epidemiology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100021, China.

Virology journal

|June 1, 2025

概括

来自宫细胞学和活检的人类乳头瘤病毒 (HPV) 基因型鉴定显示出强烈的协议,支持细胞学的临床使用. 损伤严重程度和疫苗接种情况不会显著影响HPV测试一致性.

科学领域:

在瘤学瘤学.
病毒学病毒学
遗传学遗传学是一个

背景情况:

宫癌查依赖HPV基因定型进行临床管理.
细胞学样本提供了可访问性,而活检直接与子宫病变有关.
了解这些样本类型之间的共识对于诊断可靠性至关重要.

研究的目的:

评估宫细胞学样本和FFPE活检之间的HPV基因型的一致性.
为了评估病变严重程度和疫苗接种状态对HPV基因型化协议的影响.

主要方法:

分析了392名患有宫内皮质瘤或癌症的患者的配对细胞学和FFPE活检样本.
使用SPF10-DEIA-LiPA25系统进行了HPV基因型鉴定.
使用kappa统计数据测量了同意的程度.

主要成果:

细胞学和活检HPV基因型鉴定之间的总体一致性超过95%,kappa值表明"强"到"几乎完美"的一致性.
七种HPV基因型在样本类型之间显示了统计学上不同的检测率.
多种类型的感染在细胞学中更为普遍,而单种类型的感染在活检中更为普遍.
损伤严重程度和疫苗接种状况对基因定型协议的影响最小.

结论:

来自宫细胞学和活检的HPV基因型鉴定显示出高一致性,验证了细胞学样本的临床实用性.
细胞学HPV基因型鉴定可用于临床决策,无论病变的严重程度或疫苗接种情况如何.
样本类型之间多类型和单类型感染检测的差异需要在研究中考虑.

关键词:

剥落的细胞剥落的细胞在FFPE活检中,基因型化协议协议人类乳头瘤病毒的人类乳头瘤病毒疫苗接种疫苗接种

更多相关视频

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

Published on: March 11, 2014

相关概念视频

Cancer Vaccines

Cancer Vaccines

Cancer treatment vaccines are a rapidly evolving field that offers a promising approach to immunotherapy. Unlike traditional vaccines that prevent diseases, cancer treatment vaccines are designed to treat existing cancers by stimulating the immune system to recognize and attack cancer cells.
Cancer vaccines come in two categories: preventive (prophylactic) and treatment (active). Preventive vaccines, such as the Human Papillomavirus (HPV) vaccine, protect against viruses that cause certain...