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相关概念视频

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Types of RNA01:20

Types of RNA

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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
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Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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相关实验视频

Updated: Sep 19, 2025

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
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Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

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对长非编码RNA在Solanaceae植物中的功能进行多维分析.

Wenjing Yang1,2, Quanzi Bai1, Xuan Zhang1

  • 1CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Yunnan Key Laboratory of Crop Wild Relatives Omics, State Key Laboratory of Plant Diversity and Specialty Crops, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, 650223, China.

The New phytologist
|June 2, 2025
PubMed
概括

这项研究识别和表征了七种Solanaceae物种超过113,000个长非编码RNA (lncRNA) 基因. 这些发现为探索植物 lncRNA 在发育和应激反应中的功能提供了基础资源.

关键词:
在 LncRNAs 中.这是Solanaceae.表观基因组是表观基因组的组成部分.果实成熟成熟的时间功能性注释功能性注释

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Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
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Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
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相关实验视频

Last Updated: Sep 19, 2025

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
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Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
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Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

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科学领域:

  • 植物分子生物学 植物分子生物学
  • 基因组学就是基因组学.
  • 文字转录学 (Transcriptomics) 是一个学科.

背景情况:

  • 长非编码RNAs (lncRNAs) 越来越多地被认为是植物生物学中的关键调节者.
  • 尽管识别取得了进展,但Solanaceae家族中的lncRNAs的功能注释仍然有限.

研究的目的:

  • 系统地识别和描述七种Solanaceae物种中的IncRNAs.
  • 为植物 lncRNAs 的功能注释和发现提供一个全面的资源.

主要方法:

  • 大规模链特异性RNA测序 (ssRNA-seq) 用于统一的lncRNA识别.
  • 分析包括序列,保存,表达特征,表观遗传信号和遗传突变.
  • 生物信息学方法用于功能预测和用蛋白质编码基因进行比较分析.

主要成果:

  • 大约有113,700个lncRNA基因被识别和表征.
  • 在番茄中,97.4%的lncRNAs获得了基本注释,其中25.7%预测在压力,发育和新陈代谢中的作用.
  • 与蛋白质编码基因相比,IncRNAs的独特特征得到了突出强调,1158个IncRNA与水果发育和成熟有关.

结论:

  • 这项研究为Solanaceae中的lncRNA功能研究建立了全面的框架.
  • 精心策划的数据集是了解植物过程中的lncRNA作用的宝贵资源.
  • 这些发现有助于在植物中发现新的ncRNA功能.