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猪胰腺脂酶A₂的高效生物合成在工程 Komagataella phaffii 中

Ting Zhang^1,2,3, Yijuan Xu⁴, Mengyao Wang⁴

¹Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.

Journal of agricultural and food chemistry

|June 2, 2025

概括

研究人员设计了Komagataella phaffii酵母,以有效地产生猪胰腺脂酶A2 (ppPLA2). 这种优化的菌株实现了明显更高的ppPLA2活性,为酶生产提供了可扩展的解决方案.

科学领域:

生物技术是生物技术.
酶学是一种酶学.
微生物工程微生物工程

背景情况:

脂酶A2 (PLA2) 是一个关键的酶家族,参与生理过程.
猪胰腺PLA2 (ppPLA2) 的高效生物合成对于各种应用至关重要.

研究的目的:

设计Komagataella phaffii作为一个用于高水平ppPLA2生物合成的细胞工厂.
在K. phaffii. 中优化ppPLA2表达和分泌.

主要方法:

一个分泌信号库 (SP-Lib) 的建设和选.
遗传修饰包括VPS10淘汰赛和ERO1过度表达.
优化ppPLA2基因的剂量.
在3L生物反应器中进行扩展.

主要成果:

确定了对ppPLA2表达的最佳AOX1p促进体和EXG1-pro信号.
通过基因工程实现了ppPLA2活动的7倍增加.
在生物反应器中达到1.83 × 10^5 U/L的高ppPLA2活性,比摇瓶水平增加了70倍.

结论:

在K. phaffii.中开发了一种高水平ppPLA2生物合成的有效策略.
这种工程菌株显示了工业规模ppPLA2生产的巨大潜力.
为使用微生物细胞工厂生产酶提供了有价值的见解.

关键词:

科玛格塔贝拉 (Komagataella phaffii) 是一个有趣的植物.分子的陪伴者是分子的陪伴者.脂酶A2是一种酸酶.真空分类受体的真空分类受体

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