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马斯科尼通过激活PKA/RHOA/MLC通路来降低脑内皮屏障的OGD/R诱导的超透性

Ziteng Yang¹, Yuanqi Zuo², Guangyun Wang²

¹Department of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, China.

Current neurovascular research

|June 2, 2025

概括

马斯科尼通过减少细胞死亡和改善紧密结合的完整性来保护大脑内皮屏障. 这种传统中医成分激活了PKA/RHOA/MLC通路,为治疗缺血性中风提供了潜在的机制.

科学领域:

神经科学是一个神经科学.
药理学药理学是指药理学的学科.
细胞生物学细胞生物学

背景情况:

大脑内皮屏障对大脑功能至关重要,在缺血性中风中受到损害.
香是香的关键成分,在中国传统医学中用于中风治疗,但其机制尚不清楚.
这项研究调查了muscone对大脑微血管内皮细胞 (BMEC) 对氧-葡萄糖剥夺/再输液 (OGD/R) 损伤的保护作用.

研究的目的:

为了探索马斯科尼对OGD/R诱导的内皮屏障破坏的保护作用.
为了确定muscone作用的潜在分子机制.

主要方法:

通过MTT,LDH测定,西部斑点和霍克斯特染色来评估BMEC损伤和亡.
紧结蛋白表达和局部化使用西部斑点,免疫光和法洛伊丁染色进行评估.
屏障完整性通过TEER和光素透度测量;通过分子对接,DARTS和CETSA验证路径激活.

主要成果:

马斯科尼减少了OGD/R诱导的BMEC亡和紧结蛋白质降解.
通过促进ZO-1膜表达和增加TEER,Muscone恢复了内皮屏障的完整性.
机械学研究表明,肌肉激活了PKA/RHOA/MLC通路,这对于维持内皮屏障功能至关重要.

结论:

马斯科尼抑制BMEC亡并改善内皮屏障功能,为缺血性中风提供潜在的治疗机制.
通过muscone激活PKA/RHOA/MLC通路是减少大脑内皮屏障中超透性的关键.

关键词:

我们的BMEC是BMEC.马斯克是一种.在PKA/RHOA/MLC路径中.这是TJ蛋白质.活动性细胞骨的细胞骨.灭症 (apoptosis) 是一种死亡的过程.细胞内膜屏障的障碍物缺血性中风中风

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