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相关概念视频

Polyprotic Acids03:38

Polyprotic Acids

Acids are classified by the number of protons per molecule that they can give up in a reaction. Acids such as HCl, HNO3, and HCN that contain one ionizable hydrogen atom in each molecule are called monoprotic acids. Their reactions with water are:
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
pH Regulation in Cells01:28

pH Regulation in Cells

pH plays a critical role in maintaining normal cellular activities. It helps maintain the structure and function of various proteins, dictates the charge on cellular membranes, and is crucial for metabolic reactions inside the cell. Moreover, cells use the energy from the proton motive force to generate ATP.
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
Extraction: Effects of pH00:53

Extraction: Effects of pH

Consider a neutral form of an amine, B, with a partition coefficient, K, in a liquid mixture containing organic and aqueous phases. The pH of the aqueous phase affects the charge on acidic and basic solutes, and the charged form is usually more soluble in the aqueous phase. Suppose the conjugate acid form of the amine is soluble only in the aqueous phase while the base form is soluble in both phases. Then the distribution coefficient, D, can be given as the ratio of amine concentration in the...
Conjugation01:19

Conjugation

Conjugation is a form of horizontal gene transfer that primarily occurs in bacteria and some archaea, promoting genetic diversity and adaptation. Bacteria can acquire resistance genes through conjugative plasmids, allowing them to survive antibiotic treatments that would otherwise be lethal. This process involves direct contact between cells through specialized structures such as the sex pilus and is mediated by conjugative plasmids, including the F (fertility) factor.Conjugation requires...

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相关实验视频

Updated: Jun 13, 2026

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
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聚乙烯胺中的pH依赖性结构变化影响其基因转移效率.

Shivalika Sharma1, Manjari Mishra1, Aswin T Srivatsav1

  • 1Department of Chemistry, Indian Institute of Technology Bombay, Powai, Mumbai, 400076, India.

Small (Weinheim an der Bergstrasse, Germany)
|June 2, 2025
PubMed
概括

在基因传递之前,在pH 1.4下对聚乙烯胺 (PEI) 进行调节,可以显著提高转染效率. 然而,暴露于pH3.0会导致PEI自我组装,损害基因传递和细胞吸收.

关键词:
基因转移的转移是基因的转移.散发光的光线散射器对于pH的响应性聚乙烯胺基聚乙烯是什么?自动组装的自动组装机

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科学领域:

  • 生物材料科学 生物材料科学
  • 基因传递系统是基因传递系统.
  • 聚合物化学 聚合物化学

背景情况:

  • 聚乙烯胺 (PEI) 是用于基因传递的广泛使用的非病毒载体.
  • PEI的有效性与其依赖pH的质子海绵机制有关.
  • 在低pH环境中,PEI可以自我组装成特定的结构,这可能会影响基因传递.

研究的目的:

  • 调查自由PEI链的pH条件与随后的基因转染效率之间的相关性.
  • 阐明pH诱导的PEI自我组装如何影响基因传递动态.
  • 为了确定基于PEI的基因转染的最佳pH条件和时间参数.

主要方法:

  • 分支PEI链在不同的pH值 (例如pH1.4,pH3.0) 的水溶液中进行调节,然后在pH7.4的pH值与DNA复合.
  • 在条件化后,基因转染效率得到了量化.
  • 使用对结构变化 (隐含) 敏感的技术分析了PEI自组装和微结构.

主要成果:

  • 在DNA复杂化之前以pH 1.4调节PEI,转染效率大约增加了两倍.
  • 在pH值3.0条件下的PEI链表现出转染效率的时间空间恶化.
  • 观察到PEI在pH值2.5-4.0之间自组装成微米大小的纤维,导致细胞吸收减少和核定位.

结论:

  • 自由PEI链的pH环境和调节时间极大地影响了基因转染结果.
  • 在中等酸性pH (2.5-4.0) 时PEI自组装成纤维,对基因传递有害.
  • 了解和控制PEI的pH依赖性行为对于优化非病毒基因传递系统至关重要.