相关实验视频

Updated: Jul 18, 2026

Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

Published on: October 1, 2012

在大肠炎小鼠模型中减少德克斯硫酸干扰基因表达量化

Drake Hechter¹, Sara V Good^1,2

¹Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB, CanadaR3T 2N2.

Journal of animal science

|June 3, 2025

概括

化 (LiCl) 沉有效地消除了小鼠结肠RNA中德克斯硫酸盐 (DSS) 的干扰,提高了基因表达分析的准确性. 这种方法提高了研究肠道炎症的定量实时聚合酶连锁反应 (qPCR) 的可靠性.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
免疫学免疫学免疫学

背景情况:

德克斯硫酸 (DSS) 被广泛用于诱导动物模型中的实验性肠炎症.
DSS可以干扰基因表达分析,特别是定量实时聚合酶链反应 (qPCR),影响实验结果.
准确的RNA隔离和抑制剂的消除对于在DSS治疗的组织中进行可靠的基因表达研究至关重要.

研究的目的:

为了评估化 (LiCl) 沉在从小鼠结肠RNA中去除DSS诱导的qPCR干扰方面的有效性.
为了比较标准RNA提取套件的性能,有或没有LiCl沉.
评估LiCl沉对精确测量炎症基因表达的影响.

主要方法:

从使用RNeasy Plus通用迷你套件的DSS治疗和对照小鼠的结肠组织中分离出RNA.
一个样本的子集经历了额外的化 (LiCl) 沉.
进行定量实时聚合酶链反应 (qPCR) 来分析参考基因表达和炎症标记物 (Il-6,Tnf).

主要成果:

标准的RNeasy Plus通用迷你套单独不足以消除DSS干扰,显示量化周期 (Cq) 值增加和参考基因变异.
液沉恢复了Cq值以控制水平,并减少了结肠RNA的变化.
液沉改善了检测DSS诱导的炎症标志物Il-6和Tnf在结肠组织中的上调.

结论:

将RNeasy Plus通用迷你套件与LiCl沉相结合,对于在DSS处理的小鼠结肠组织中进行可靠的基因表达分析至关重要.
液化沉有效消除了DSS干扰,提高了qPCR准确性和炎症反应的检测.
这种优化的方法提高了涉及DSS诱导肠道炎症的实验测试的功率.

关键词:

德克斯酸硫酸盐是什么意思基因表达的基因表达方式炎症性肠病是一种炎症性肠病.干扰干扰是干扰的它们是mRNARNA.

更多相关视频

Effects of Taste Signaling Protein Abolishment on Gut Inflammation in an Inflammatory Bowel Disease Mouse Model

Effects of Taste Signaling Protein Abolishment on Gut Inflammation in an Inflammatory Bowel Disease Mouse Model

Published on: November 9, 2018

Investigating Target Gene Function in a CD40 Agonistic Antibody-induced Colitis Model using CRISPR/Cas9-based Technologies

Investigating Target Gene Function in a CD40 Agonistic Antibody-induced Colitis Model using CRISPR/Cas9-based Technologies

Published on: June 2, 2021

相关概念视频

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...