单细胞分辨率揭示了邻近的细胞亚型,在胎儿丸发育过程中共享类固醇生成能力
Keer Jiang1,2, Zirui Fu1, Philippos Tsourkas3
1Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706.
概括
胎儿莱迪格细胞表现出基因表达异质性,有些细胞缺乏完整的类固醇基因组. 最佳的雄激素生物合成可能是小鼠丸发育过程中这些细胞之间的合作努力的结果.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 发育生物学是发展生物学.
背景情况:
- 从历史上看,内分泌细胞被认为是统一协调的.
- 最近的单细胞研究揭示了内分泌细胞群体内的异质性.
- 单个细胞可以作为独立调节的单元发挥作用.
研究的目的:
- 在小鼠丸发育过程中调查胎儿莱迪格细胞对雄激素生产的贡献.
- 分析基因表达异质性及其对类固醇生成的影响.
- 确定雄激素生物合成是否是一种集体或个体细胞过程.
主要方法:
- 单分子光 in situ 杂交 (sm-FISH) 用于基因表达分析.
- 单细胞RNA测序 (scRNA-seq) 用于细胞类型识别和基因分析.
- 对雄激素水平和类固醇基因表达的时间和空间分析 (Star,Cyp11a1,Cyp17a1,Hsd3b1).
主要成果:
- 类固醇基因表达的峰值先于雄激素生产的峰值.
- 类固醇细胞在发育过程中表现出动态的空间分布.
- 胎儿莱迪格细胞显示非同步的基因转录和处理.
- 一些细胞表达不完整的类固醇路径基因组.
- scRNA-seq和sm-FISH在各种间歇细胞中证实了不完整的基因组.
结论:
- 胎儿类固醇基因表达受到严格监管.
- 转录的存在在个体胎儿莱迪格细胞之间有所不同.
- 最佳的雄激素生物合成可能来自邻近的类固醇细胞之间的合作相互作用.
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