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在儿童结核病中进行Xpert Ultra测试的便处理方法:一个前性的,多国准确性研究.
Devan Jaganath1,2, Pamela Nabeta3, Mark P Nicol4
1Division of Pediatric Infectious Diseases, University of California, San Francisco, San Francisco, USA.
使用Xpert Ultra进行儿童结核病 (TB) 检测的无离心机便处理方法被发现是可以接受和可用的. 培养阳性病例的敏感性最高,显示了改善儿童结核病诊断的潜力.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 儿科健康 儿科健康
背景情况:
- 在儿童结核病 (TB) 中进行Xpert Ultra便测试的无离心机方法正在出现,但缺乏全面的准确性和可用性数据.
- 由于非特异性症状和样本采集困难,对儿童结核病的准确诊断具有挑战性.
研究的目的:
- 评估三种无离心机便处理方法 (便处理套件[SPK],简单的一步[SOS],优化糖漂浮[OSF]) 的准确性,可接受性和可用性,用于Xpert Ultra测试可能患有结核病的儿童.
- 将这些方法的性能与微生物学和复合材料参考标准进行比较.
主要方法:
- 这是一项前性研究,涉及印度,南非和乌干达15岁以下的607名可能患有结核病的儿童.
- 便样本使用SPK,SOS和OSF方法进行处理,用于Xpert Ultra测试.
- 根据微生物学参考标准 (MRS) 和复合参考标准 (CRS) 进行准确性评估. 实验室工作人员调查评估了方法的可接受性和可用性.
主要成果:
- 对MRS的敏感性在31.3% (OSF) 到38.6% (SOS) 之间,具有很高的特异性 (97.1%98.2%).
- 所有的方法都被认为是可以接受和可用的,SOS被认为是对外围设施最可行的.
- 在培养阳性儿童中,敏感性显著改善 (55.0%77.3%),并在便Xpert Ultra添加到唾液检测时增加 (0%11.8%的改善).
结论:
- 对于Xpert Ultra测试的离心机无便处理方法是可以接受的,并且可以用于儿童结核病诊断.
- 这些方法表现相似,在培养阳性儿科结核病病例中观察到的最高灵敏度.
- 这些发现支持这些方法在资源有限的环境中对改善儿童结核病检测的潜在实用性.
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