在Ca2+通道CNGC2和CNGC20中介于甲基莉酸诱导的信号传递和寒冷耐受性
Yanliang Guo1,2, Jiayue Li1, Lingling Liu1
1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China.
Plant physiology
|June 3, 2025
概括
甲基斯酸盐 (MeJA) 通过通过ClCNGC2和ClCNGC20通道增加 (Ca2+) 流入来提高植物的耐寒性. 这些通道相互激活,导致水平升高,植物抗寒能力提高.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 压力生理学 压力生理学
背景情况:
- 植物耐寒应激对农业至关重要.
- 已知植物激素甲基斯酸盐 (MeJA) 可以增强耐寒性,但机制尚不清楚.
- 信号传递在植物应激反应中起作用.
研究的目的:
- 阐明MeJA增强西瓜 (Citrullus lanatus) 耐寒性的分子机制.
- 研究离子通道在MeJA介导的冷应激反应中的作用.
- 为了确定参与MeJA信号通路的关键基因,用于冷耐受性.
主要方法:
- 在西瓜和Arabidopsis thaliana中的基因沉默和过度表达.
- 测量细胞质中自由的Ca2+ ([Ca2+]cyt) 度.
- 基因表达的分析,包括循环核酸门离子通道 (ClCNGC) 基因和C-REPEAT结合因子 (CBF) 途径基因.
- 寒冷压力治疗和耐受性测试.
主要成果:
- 在西瓜中,MeJA诱导了一次暂时的Ca2+流入,并在寒冷压力期间提升[Ca2+]细胞.
- 在暴露在寒冷中时,MeJA可以调节两个Ca2+透通道基因,即ClCNGC2和ClCNGC20.
- 沉默或过度表达ClCNGC2/ClCNGC20会影响Ca2+流入,CBF通路激活和耐寒性.
- ClCNGC2和ClCNGC20表现出相互的转录激活.
结论:
- 通过通过相互依赖的ClCNGC2和ClCNGC20通道系统调解Ca2+流入,MeJA提高了植物的寒冷耐受性.
- 这一过程导致[Ca2+]cyt升高,随后CBF通路激活,并改善结耐受性.
- 这些发现为MeJA诱导的寒冷耐受性提供了分子洞察力,并为培育耐寒作物提供了潜力.
相关概念视频
01:16Calmodulin-dependent Signaling
5.3K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.3K
02:45Responses to Heat and Cold Stress
13.9K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.9K
01:27C4 Pathway and CAM
46.3K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
C4 Pathway
The C4 pathway is used by plants such as...
46.3K
01:25Cell Signaling in Plants
5.8K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.8K
01:12Mechanically-gated Ion Channels
6.7K
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
6.7K
01:27Feedback Regulation of Calcium Concentration
3.5K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.5K