相关实验视频
Updated: Sep 19, 2025

07:20
Author Spotlight: Imaging ATG9A, a Multi-Spanning Membrane Protein
Published on: June 16, 2023
2.3K
ATG9A 和 ARFIP2 合作控制 lysosomal 修复的 PI4P 水平
Stefano De Tito1, Eugenia Almacellas1, Daniel Dai Yu1
1Molecular Cell Biology of Autophagy Laboratory, The Francis Crick Institute, London, UK.
Developmental cell
|June 3, 2025
概括
调动的ATG9A囊泡将PI4K2A传递给受损的溶解体,防止细胞死亡. 在这些囊泡上,ARFIP2平衡脂质转移,并有助于囊泡检索,在损伤和感染期间保持溶酶体平衡.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 溶酶体损伤可以触发细胞死亡途径.
- 细胞内膜网膜 (ER) - 溶酶体接触部位参与了溶酶体修复.
- 酸丁醇4-酶-2a (PI4K2A) 对于这种修复至关重要,但其传递到受损的溶解体尚不清楚.
研究的目的:
- 为了研究PI4K2A传递到受损 lysosomes的机制.
- 阐明ATG9A囊泡在 lysosome 修复中的作用.
- 了解ARFIP2在溶酶体平衡中的功能.
主要方法:
- 细胞成像和显微镜技术.
- 对ATG9A和ARFIP2.2.进行基因操纵.
- 对脂质转移蛋白和膜接触部位的分析.
主要成果:
- 含有ATG9A的囊泡在无菌损伤和细菌感染期间将PI4K2A传递给受损的溶酶体.
- ARFIP2在溶酶体上结合和隔离PI4P,调节脂质的转移.
- ARFIP2招募了AP-3,促进了ATG9A囊泡的获取.
结论:
- 调动的ATG9A囊泡对于将PI4K2A输送到受损的溶解体至关重要.
- 在平衡溶酶体修复和囊泡贩运方面,ARFIP2发挥着关键作用.
- 这些发现揭示了在损伤和感染后维持 lysosome 稳态的新机制.
相关概念视频
Coat Assembly and GTPases
3.6K
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
3.6K
Rab Cascades
2.7K
Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
2.7K
Export of Misfolded Proteins out of the ER
3.9K
After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
3.9K
DNA Damage can Stall the Cell Cycle
9.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.3K
Long-patch Base Excision Repair
7.2K
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
7.2K
Rab Proteins
4.1K
Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
4.1K

