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Western Blotting

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

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相关实验视频

Updated: May 1, 2026

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs

Published on: January 27, 2021

通过蛋白质学区分蛋:来自中国北方西图的一个案例研究.

Binjie Qi¹, Haifeng Dou², Wei Liu²

¹Department of Archaeology and Anthropology, University of Chinese Academy of Sciences, Beijing 100049, China.

Journal of proteomics

|June 3, 2025

概括

古蛋白质组学成功地从中国发现了古老的灰色卵,使用新标记将它们与天区分开来. 这为早期 domestication和文化实践提供了分子证据.

关键词:

蛋蛋的外表古蛋白质组学是古蛋白质组学.稳定的同位素是稳定的.在Xitou网站上.动物园动物园MS ZooMS

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Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo

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Separation of Avian Preovulatory Follicle Granulosa and Theca Cell Layers for Downstream Applications

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Published on: October 25, 2024

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Last Updated: May 1, 2026

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs

Published on: January 27, 2021

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Published on: April 21, 2022

Separation of Avian Preovulatory Follicle Granulosa and Theca Cell Layers for Downstream Applications

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科学领域:

古蛋白质组学是古蛋白质组学.
动物园考古学
同位素分析分析同位素分析

背景情况:

蛋具有文化意义,但在考古学中很难在物种层面上识别.
传统的方法和现有的质谱技术难以区分像Anser cygnoides和Anser anser.等密切相关的物种.
准确的蛋识别对于了解过去的人与动物的互动和文化习惯至关重要.

研究的目的:

开发和应用新的古蛋白质学方法来区分安塞和安塞蛋.
为了识别来自中国西图考古遗址的蛋碎片的物种.
调查与这些蛋相关的的化状态和饮食.

主要方法:

用质谱法 (ZooMS) 进行动物园考古学,用于蛋白型.
液体染色学-并联质谱学 (LC-MS/MS) 用于标记分析.
碳同位素分析和外厚度测量.

主要成果:

鉴定出了新的标记物和氨基酸变体,可靠地区分安赛尔cygnoides和安赛尔 anser.
来自Xitou遗址的三个蛋标本被确定为灰色 (Anser anser).
同位素分析表明饮食主要是C3植物,外厚度表明可能的化.

结论:

这项研究提供了首个直接的分子证据,证明可能在中国养了灰.
这些发现为家禽养,古代农业实践以及在中国的文化意义提供了新的见解.
开发的蛋白质学方法提高了考古蛋碎片的识别能力.