抗循环赫西米德的核糖体显示了 C.C. 的适应性翻译动态. 伊莱根斯 (elegans) 是一个词
bioRxiv : the preprint server for biology
|June 4, 2025
概括
核糖体蛋白RPL-36A的一种新型突变赋予了对蛋白转化抑制剂循环赫西米德 (CHX) 的抗性. 这一发现有助于研究翻译调节,并揭示出意想不到的发育时间变化.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 发展生物学 发展生物学
背景情况:
- 蛋白质翻译调节对于细胞功能和发育至关重要.
- 了解翻译延长阶段的中断是至关重要的,但不完整的.
- 核糖体蛋白修饰在细胞反应中的作用需要进一步研究.
研究的目的:
- 识别和描述影响蛋白质翻译延长的突变.
- 探索翻译延长抑制对细胞过程和发育的影响.
- 开发用于对核糖体蛋白的基因操纵的新工具.
主要方法:
- 在*Caenorhabditis elegans*中基于CRISPR的基因组编辑
- 识别对循环赫西米德 (CHX) 耐药的核糖体蛋白突变 (RPL-36A P55Q)
- 核糖体概况分析用于分析翻译动态.
- 应力通路激活的分析 (RQC,RSR,ISR)
主要成果:
- 在RPL-36A中单个氨基酸替代 (P55Q) 赋予了完全的CHX耐药性.
- 异卵性RPL-36A(P55Q) 突变体呈现正常发育,具有中等的CHX耐药性.
- RPL-36A(P55Q) 作为CRISPR编辑核糖体蛋白基因的可选择标记物.
- 延长抑制导致基因特异性的翻译效率变化,而不是正规的应激反应.
- 部分翻译抑制促进过早的 oogenesis.
结论:
- RPL-36A ((P55Q) 是研究翻译延长和开发基因组编辑策略的宝贵工具.
- 抑制翻译延长可以通过改变翻译效率来微调发育时间.
- 长期的翻译延长抑制并不能普遍激活细胞应激通路.
相关概念视频
Termination of Translation
25.3K
The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...
25.3K
Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Improving Translational Accuracy
9.6K
Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
9.6K
Translation
14.7K
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Proteins are...
Translation Produces the Building Blocks of Life
Proteins are...
14.7K
Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K


