通过益生菌Escherichia coli进行表皮性HIF的调节
bioRxiv : the preprint server for biology
|June 4, 2025
概括
大肠杆菌在肠道中激活缺氧诱导因子 (HIF),促进肠道屏障功能. 这个过程依赖于细菌呼吸,可能为炎症性肠病 (IBD) 提供新的治疗方法.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 细胞生物学 细胞生物学
背景情况:
- 肠道微生物群影响宿主生理学,包括免疫和神经功能.
- 由肠道微生物产生的短链脂肪酸 (SCFA) 诱导生理缺氧,稳定缺氧诱导因子 (HIF) 并促进肠道屏障功能.
- 众所周知,致病性细菌可以独立于SCFA调节HIF,但对于共生物种来说,这一点尚未确立.
研究的目的:
- 为了研究非致病性,开始性大肠杆菌菌株是否可以稳定肠道上皮细胞中的HIF.
- 为了确定HIF激活由共生大肠杆菌驱动一个亲障碍转录程序.
- 探索细菌有氧呼吸在这种HIF介导作用中的作用及其体内相关性.
主要方法:
- 在实验室内使用暴露于共生大肠杆菌菌株的肠上皮细胞进行的研究.
- 对大肠杆菌进行基因操纵以消除有氧呼吸.
- 对HIF稳定和转录活动的分析.
- 在野生型或呼吸缺陷的大肠杆菌 (E. coli) 殖民的小鼠身上进行的实验.
主要成果:
- 有证据表明,大肠杆菌在肠道上皮细胞中稳定了HIF.
- 激活的HIF驱动了一个支持肠道屏障功能的转录程序.
- 这种HIF稳定性取决于大肠杆菌的有氧呼吸.
- 被野生型*大肠杆菌*殖民的抗生素治疗小鼠表现出诱导的组织缺氧,与呼吸缺陷菌株的小鼠不同.
结论:
- 非致病性大肠杆菌可以通过有氧呼吸在肠表皮中激活HIF.
- 这种机制有助于肠道平衡和肠道屏障功能.
- 这些发现表明,对于IBD等炎症状况,利用益生菌*大肠杆菌*来调节HIF信号传递,这可能是一种潜在的治疗策略.
更多相关视频
08:04Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids
Published on: December 18, 2017
14.4K
10:59Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens
Published on: May 12, 2019
10.0K
相关概念视频
Stringent Response in E. coli
62
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
62
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.3K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.3K
Regulation of Angiogenesis and Blood Supply
2.7K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.7K
Other Stress Responses in Bacteria
72
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
72
Epigenetic Regulation
31.4K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
31.4K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.2K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.2K
