Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Epigenetic Regulation01:37

Epigenetic Regulation

3.0K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.0K
Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

9.8K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
9.8K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

8.2K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.2K
Heterochromatin02:38

Heterochromatin

11.5K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
11.5K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

7.3K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.3K
Position-effect Variegation02:32

Position-effect Variegation

6.3K
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
6.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Timothy syndrome and CACNA1C-Related Disorder: first international language and management guidelines consensus statement.

European journal of human genetics : EJHG·2026
Same author

Stabilizing and strengthening the US physician-scientist faculty workforce in academic medicine: a proposed institutional framework.

JCI insight·2026
Same author

Zebrafish otic vesicle and mouse epididymis as model systems for studying columnar epithelial cell division.

Scientific reports·2026
Same author

JACC: Basic to Translational Science: Honoring the Top Reviewers of 2024.

JACC. Basic to translational science·2025
Same author

Timothy Syndrome and CACNA1C-Related Disorder: First International Language and Management Guidelines Consensus Statement.

Research square·2025
Same author

FGF13 is not secreted from neurons.

bioRxiv : the preprint server for biology·2025

相关实验视频

Updated: Jun 12, 2025

A Method to Study de novo Formation of Chromatin Domains
00:07

A Method to Study de novo Formation of Chromatin Domains

Published on: August 23, 2019

5.4K

宏分子相互作用决定了Polycomb介导的表观遗传抑制.

Christian Much1,2, Sandy M Rajkumar1,2,3, Liming Chen1,2,3

  • 1Cardiovascular Research Institute, Weill Cornell Medicine, New York, NY 10021, USA.

bioRxiv : the preprint server for biology
|June 4, 2025
PubMed
概括

多胞体抑制复合体2 (PRC2) 亚复合体在基因抑制和干细胞分化中具有不同的作用. 具体的相互作用揭示了PRC2如何调节心肌细胞的发育和功能.

更多相关视频

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes
10:26

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes

Published on: January 16, 2015

8.4K
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.4K

相关实验视频

Last Updated: Jun 12, 2025

A Method to Study de novo Formation of Chromatin Domains
00:07

A Method to Study de novo Formation of Chromatin Domains

Published on: August 23, 2019

5.4K
Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes
10:26

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes

Published on: January 16, 2015

8.4K
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.4K

科学领域:

  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 分子生物学分子生物学
  • 发展生物学 发展生物学

背景情况:

  • 动态表观遗传调节涉及复杂的宏分子相互作用.
  • 聚合体抑制复合体2 (PRC2) 沉积H3K27me3,形成不同的子复合体 (例如,PRC2.1,PRC2.2),其功能不清楚,可能是多余的.

研究的目的:

  • 阐明PRC2亚复合体在谱系特异性基因表观遗传抑制中的独特作用.
  • 了解在人类干细胞分化过程中,特别是心肌细胞发育过程中,PRC2亚复合体的特定功能.

主要方法:

  • 在人类多能干细胞模型中设计了功能分离突变体.
  • 在PRC2亚复合体内剖析单个蛋白质-蛋白质和DNA-蛋白质相互作用.
  • 分析了特定位置的H3K27me3沉积及其对基因表达和分化的影响.

主要成果:

  • PRC2.1和PRC2.2表现出特定于位点的H3K27me3沉积,导致对心肌细胞分化产生相反的影响.
  • MTF2-PRC2.1通过DNA和H3K36me3相互作用刺激干细胞中的抑制和心脏分化.
  • PHF19对抗MTF2-PRC2.1活动,而MTF2-PRC2.1对正常心肌细胞功能至关重要.

结论:

  • 在表观遗传基因抑制中,PRC2亚复合体具有特定的,非冗余的功能.
  • 在PRC2内部的单个宏分子相互作用对于人类干细胞的精确Polycomb介导表观遗传调节和分化至关重要.
  • 了解这些特定的相互作用是解读发育过程中复杂表观遗传控制的关键.