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在细胞动力学过程中,Ync13-Rga7-Rng10复合体对分泌囊泡向的空间控制

Sha Zhang¹, Davinder Singh¹, Yi-Hua Zhu¹

¹Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA.

bioRxiv : the preprint server for biology

|June 4, 2025

概括

一个保存的蛋白质模块在细胞分裂过程中精确地准囊泡,确保正确的隔膜形成和细胞完整性. 这一发现揭示了细胞运动中的膜流通控制.

科学领域:

细胞生物学细胞生物学
分子和细胞生物学分子和细胞生物学.
生物化学生物化学

背景情况:

细胞动力学或细胞分裂需要协调的膜运输来实现细胞完整性.
在细胞动力学过程中控制囊泡向的机制仍然不完全理解.
参与细胞运动的关键蛋白质在真核生物中被保存.

研究的目的:

为了确定调节裂变酵母细胞动力学过程中囊泡向的分子机制.
为了阐明特定蛋白质在分裂中的膜贩运中的作用.

主要方法:

分裂酵母遗传学分裂酵母遗传学
光显微镜的光学显微镜.
在体外结合测定试验中的结合测定.

主要成果:

鉴定了一种在细胞动力学过程中对囊泡准至关重要的保存模块 (Ync13,Rga7,Rng10).
这个模块招募TRAPP-II复合体,将葡萄糖合成酶 (Bgs4,Ags1) 输送到裂解.
Ync13与Sec1进行囊泡融合,突变破坏隔膜完整性,导致细胞溶解.

结论:

Ync13-Rga7-Rng10模块在细胞动力学过程中确保精确的囊泡向和融合.
这一途径对于通过适当的隔膜形成来维持细胞完整性至关重要.
提供了关于细胞分裂中的膜贩运的时空调节的新见解.

关键词:

在Ags1中,Ags1是Ags1.Bgs4 Bgs4 Bgs4 Bgs4 Bgs4 Bgs4 Bgs4 Bgs4 Bgs4 Bgs4 Bgs4 第一节第一节第一节这是一个微笑.这是TRAPP-II综合体.细胞动力学细胞动力学细胞动力学分裂酵母酵母的分裂酵母是什么隔膜 septum 隔膜是指一个隔膜.

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