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BONCAT-Live用于隔离和培养活跃的环境微生物

Sayali A Mulay^1,2, Tatiana A Vishnivetskaya², Leah H Hochanadel¹

¹Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.

bioRxiv : the preprint server for biology

|June 4, 2025

概括

科学家们开发了BONCAT-Live,以隔离应对环境变化的特定微生物. 这种方法在北极永久土和树层中发现了活跃的细菌,这些微生物在植物化合物上壮成长.

科学领域:

微生物学与环境科学微生物学与环境科学
大基因组学和微生物生态学

背景情况:

微生物是全球生物地球化学循环和物种间相互作用的重要驱动因素.
微生物生理状态是动态的,通过代谢变化对环境变化做出反应.
目前的分子方法可以识别微生物的反应,但很难分离特定的响应细胞.

研究的目的:

开发和实施一种新的方法来隔离特定的微生物细胞,这些细胞会对环境刺激做出反应.
从不同的环境中识别和培养适应特定条件的微生物.

主要方法:

综合生物对等非规范性氨基酸标记 (BONCAT) 与细胞隔离和培养技术.
应用BONCAT-Live到各种原生微生物群落,包括北极永久土,植物根球和人类口腔微生物群.
培养的微生物细胞对环境线索表现出特定的反应,如解,营养丰富和宿主/微生物代谢物.

主要成果:

从北极永久土中成功识别和分离了眠的细菌,这些细菌在解时被激活.
孤立的植物根系微生物在根排泄物上壮成长,充当营养和防御化合物.
鉴定和分离的人类口腔细菌,在对宿主或同时发生的微生物代谢物反应中迅速繁殖.

结论:

根据其功能反应,BONCAT-Live是一种有效的方法,可以从复杂的本地社区中分离特定的微生物细胞.
这种方法有助于对微生物适应环境变化的机制进行深入的描述.
为了解微生物在各种生态系统中的作用及其相互作用提供了一个强大的工具.

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