相关实验视频

Updated: Jun 12, 2025

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

Published on: November 14, 2018

在使用CRISPR-Cas9的Candida albicans中向失去了异性

Philippe C Després^1,2,3, Nicholas C Gervais³, Meea Fogal³

¹Infectious Disease and Microbiome Program, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, United States of America.

bioRxiv : the preprint server for biology

|June 4, 2025

概括

研究人员开发了一种CRISPR-Cas9方法,精确地诱导Candida albicans中异构性 (LOH) 的丧失. 这项技术有助于研究遗传多样性如何驱动这种真菌病原体的抗真菌耐药性.

科学领域:

微生物学微生物学
遗传学是一个遗传学.
分子生物学分子生物学

背景情况:

* *Candida albicans*,是一种真菌病原体,具有高度异构的基因组.
*遗传多样性,特别是异构性 (LOH) 的丧失,可以推动在抗真菌压力下的适应.
*精确研究特定LOH事件的表型影响是具有挑战性的.

研究的目的:

* 开发一种方法,以有针对性地诱导Candida albicans*中的LOH事件.
*为了验证LOH在抗真菌耐药性的作用.
* 为未来的LOH研究创造一个资源.

主要方法:

*采用了CRISPR-Cas9系统,其中包含异位基因特异性导向RNA序列.
*诱导向的,指导的LOH事件在 *Candida albicans*.
*使用全基因组长读测序验证了LOH事件.

主要成果:

*成功地重复了一个已知的LOH事件,赋予了流可纳耐药性.
* 证明诱导的LOH重组通道长度类似于自然事件.
* 开发了一个基因组特异性sgRNA序列的数据库,用于广泛的基因组向.

结论:

*CRISPR-Cas9能够在C. albicans*中精确地诱导特定于基因的LOH.
* 这种方法有助于研究真菌病原体的适应性LOH事件.
*提供的sgRNA数据库支持全基因组的LOH研究.

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Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

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CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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Homologous Recombination

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