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卵细胞线粒体将母体环境与后代的表型联系起来

Jason F Cooper¹, Kim Nguyen¹, Darrick Gates¹

¹Van Andel Research Institute, Department of Metabolism and Nutritional Programing, Grand Rapids, Michigan, USA, 49503.

bioRxiv : the preprint server for biology

|June 4, 2025

概括

孕产妇的胰岛素信号传递和环境塑造了卵细胞的线粒体功能,特别是复合III. 这种重塑对于后代来说至关重要,通过通过AMP-激酶 (AAK-2) 改变他们的新陈代谢来适应压力.

科学领域:

生殖生物学生殖生物学
线粒体生物学线粒体生物学
代际继承权代际继承权

背景情况:

卵子细胞在成熟过程中经历了保守的线粒体电子运输链 (ETC) 重塑,由胰岛素信号调节.
这种卵细胞ETC重塑的生物学作用及其潜在的分子机制在很大程度上是未知的.
之前的工作将母亲的胰岛素信号与后代通过代谢变化适应透应激联系起来.

研究的目的:

阐明卵细胞调解代际应激反应的分子机制.
调查卵细胞ETC重塑在将母体环境与后代代谢适应联系的作用.

主要方法:

开发一个低输入的卵细胞蛋白学工作流程.
蛋白质组学与*Caenorhabditis elegans*代际透应激反应模型的整合.
对ETC复合物III功能和AMP-激酶 (AAK-2) 途径分析的遗传干扰.

主要成果:

母亲环境和胰岛素信号共同调节卵细胞ETC蛋白的丰度,特别是复合III.
在卵细胞中增强ETC复合体III功能是必要的,并且足以将母体环境与后代代谢变化联系起来.
卵细胞复合体III功能障碍通过AMP-激酶 (AAK-2) 依赖途径影响后代的新陈代谢.

结论:

卵细胞ETC重塑,特别是复杂III活动,是母体环境和后代代谢适应之间的关键联系.
母亲的环境通过调节卵细胞线粒体功能来影响后代的应激弹性.
后代中的AMP-激酶 (AAK-2) 途径调节了在压力下对ATP保存和甘油代谢的代谢调整.

关键词:

在AMP基因酶中,AMP基因酶的作用是:这里是C. elegans.综合体I 是一个综合体.第三个综合体.欧洲交易委员会 (ETC) 的这是代际的,是代际的.这就是Aak-2Aak-2.daf-2 daf-2 daf-2 daf-2 daf-2 daf-2 daf-2 daf-2 daf-2 daf-2 daf-2 daf-2 胰岛素的胰岛素胰岛素是什么这就是ISP-1 isp-1.线粒体中的线粒体.卵子细胞卵子细胞

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