相关实验视频

Updated: Sep 19, 2025

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Published on: October 15, 2018

希斯甲基转移酶SETDB1在正常和恶性血液形成中的作用

Yu-Hsuan Chang¹, Susumu Goyama¹

¹Division of Molecular Oncology, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Tokyo, Japan.

|June 4, 2025

概括

SET 域分叉的ヒ斯甲基转移酶 1 (SETDB1) 沉默基因并作为免疫检查点. SETDB1抑制天然杀手细胞对白血病的反应,突出其在癌症免疫力中的作用.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

基因组甲基转移酶1 (SETDB1) 是一个关键的基因组甲基转移酶.
它调解了可转移元素,内源逆转录病毒和免疫基因的转录沉默.
SETDB1与ATF7IP和TRIM28等共因子合作,并与DNA甲基化进行基因抑制.

研究的目的:

通过其基因组甲基转移酶活性审查SETDB1在基因调节中的作用.
讨论SETDB1的结构特征和辅助因素.
突出SETDB1在血液形成中的特异性功能及其作为血液恶性瘤中的免疫检查点的作用.

主要方法:

文献综述侧重于SETDB1在基因调节和癌症免疫中的功能.
分析SETDB1在表观遗传沉默机制中的作用.
检查SETDB1在正常的造血过程和血液恶性瘤中的参与.

主要成果:

通过H3K9甲基化,SETDB1在转录沉默中发挥作用.
SETDB1被确定为各种瘤中关键的免疫检查点基因.
SETDB1抑制自然杀手 (NK) 细胞介导的抗白血病反应.

结论:

在表观遗传基因调节中,SETDB1起着至关重要的作用.
SETDB1充当免疫检查点,影响抗瘤免疫力,特别是在血液恶性瘤中.
了解SETDB1的功能对于开发新型癌症免疫疗法至关重要.

关键词:

在SETDB1中,我们可以使用SETDB1.表观遗传学是指表观遗传学.血液形成血液形成基因组胺甲基化基因组胺甲基化这种白血病是白血病.

更多相关视频

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

相关概念视频

Histone Modification

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...

Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

Spreading of Chromatin Modifications

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...

Role of Hematopoietic Growth Factors

Role of Hematopoietic Growth Factors

Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...

Multipotency of Hematopoietic Stem Cells

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...

Hematopoiesis

Hematopoiesis

The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...