通过安波特二增强SARS-CoV-2感染:对疾病管理的影响
Dung Nguyen1,2, Stephen M Laidlaw1,2, Xiaofeng Dong3
1Centre for Human Genetics, University of Oxford, Oxford, United Kingdom.
在细胞研究中,用于真菌感染的安福特里辛B和尼斯塔丁意外地增加了SARS-CoV-2的复制. 这些抗真菌药物可能通过促进病毒进入来增强COVID-19的疾病,警告不要在活跃病例中使用它们.
科学领域:
- 病毒学 病毒学
- 菌类学 菌类学是指菌类学.
- 药理学 药理学是指药理学的学科.
背景情况:
- 严重的COVID-19患者容易患入侵性肺粘膜菌症.
- 氨酸B (AmB) 是侵袭性肺粘膜菌的主要治疗方法.
- AmB对病毒复制的影响是可变的,需要对SARS-CoV-2进行调查.
研究的目的:
- 为了研究AmB和nystatin与SARS-CoV-2蛋白质的in silico相互作用.
- 评估AMB,尼斯塔丁和其他抗真菌药物对SARS-CoV-2感染和复制的体外影响.
- 阐明AMB影响SARS-CoV-2的机制.
主要方法:
- 在 AmB 和 nystatin 对 SARS-CoV-2 蛋白质的选中.
- 在体外测试中使用Vero E6,Calu-3和Huh7细胞来评估SARS-CoV-2的复制.
- 药物动力学研究和基因淘汰实验针对干扰素诱导的跨膜蛋白 (IFITM).
主要成果:
- 在基分析预测AmB,nystatin和SARS-CoV-2蛋白质之间的强烈相互作用.
- 在体外,AmB和nystatin显著增加了SARS-CoV-2的复制 (在Vero E6细胞中高达100倍).
- 艾姆比增强病毒的进入,可能通过克服IFITM3介导的抗病毒效应.
结论:
- 与预测相反,AmB和nystatin可以增强SARS-CoV-2的感染和复制.
- 这些抗真菌药物有助于病毒进入宿主细胞.
- 由于潜在的疾病增强,AMB和nystatin不应用于活跃COVID-19患者的真菌感染.
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