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工程Corynebacterium glutamicum具有高活性血红蛋白和髓红蛋白合成的有效血红供应

Song Wang^1,2,3,4,5, Zirui Yin^2,3,4,5, Yue Fan^2,3,4,5

¹Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, 2699 Qianjin Street, Changchun, Jilin 130023, China.

Journal of agricultural and food chemistry

|June 4, 2025

概括

研究人员设计了一种Corynebacterium glutamicum菌株,用于微生物的功能性血红蛋白 (Hb) 和肌红蛋白 (Mb) 生产. 这一突破使得这些至关重要的血液蛋白质可用于各种应用的可扩展合成.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
微生物生产微生物生产

背景情况:

血球蛋白 (Hb) 和肌球蛋白 (Mb) 是重要的蛋白质,具有作为氧载体和食品添加剂的应用.
功能性Hb和Mb的微生物合成存在重大挑战,特别是在高效的血红素生产中.

研究的目的:

设计一种Corynebacterium glutamicum菌株,以有效地产生活性Hb和Mb的微生物.
为了克服血红蛋白生物合成和蛋白质表达的局限性,提高血红蛋白产量.

主要方法:

代的多模块策略,以优化血红素供应,包括前体增强和途径选择.
工程化血红素生物合成途径 (HEME3) 和血红素输出者淘汰 (ΔhrtBA).
优化了大豆Hb,三叶草Hb,牛Mb和猪Mb的表达.

主要成果:

通过代谢工程实现了血红素标位的90.75倍增加.
产生的最高报告的摇瓶标位是大豆Hb (1.10 g/L),三叶草Hb (1.12 g/L),牛Mb (0.56 g/L) 和猪Mb (1.14 g/L).
生物合成的Hb和Mb表现出与其天然对应物相比的生化特性.

结论:

开发了一个可扩展的微生物平台,使用工程Corynebacterium glutamicum生产功能性血蛋白.
证明了生产具有高产量和本地生物化学功能的多种Hb和Mb变体的可行性.

关键词:

在Corynebacterium glutamicum的研究中,我们发现荷姆供应供应荷姆提供供应血红蛋白血红蛋白是一个血液蛋白质是血液中的蛋白质.肌球蛋白是什么?肌球蛋白是什么?

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