CD163+巨细胞通过骨质质素分泌驱动快速肺纤维化
Wenjun Ding1, Suihui Deng1, Zhiping Wang1
1State Key Laboratory of Traditional Chinese Medicine Syndrome, Guangzhou University of Chinese Medicine, Guangzhou, China; Research Centre of Basic Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.
International immunopharmacology
|June 4, 2025
概括
CD163阳性巨细胞通过分泌骨质素 (OPN) 来驱动快速肺纤维化 (RPF). 针对这种CD163-巨细胞-OPN通路可能为RPF提供新的治疗方法.
科学领域:
- 肺部医学 肺部医学
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 快速肺纤维化 (RPF) 是一种致命的疾病,与严重的呼吸道感染有关,其特征是细胞外矩阵的过度积累.
- 在RPF患者中,CD163阳性巨细胞的数量越来越多,但它们的具体作用尚不清楚.
研究的目的:
- 为了研究CD163阳性巨细胞在COVID-19相关RPF的发病过程中的作用.
- 探索CD163-巨细胞-骨质素 (OPN) 轴作为RPF的治疗点的潜力.
主要方法:
- 在人类RPF样本中进行集成单细胞RNA测序 (scRNA-seq) 和单核RNA测序 (snRNA-seq).
- 使用了三次LPS诱导的小鼠RPF模型和体外共同培养系统.
- 使用遗传 (CD163淘汰) 和功能 (OPN沉默) 方法.
主要成果:
- CD163阳性巨细胞和纤维化相关基因SPP1 (编码OPN) 在COVID-19 RPF肺部中被丰富.
- 在小鼠模型中,CD163阳性巨细胞加剧了RPF,OPN水平与原沉积相关.
- 在体外,OPN沉默降低了CD163阳性巨细胞的profibrotic活性.
结论:
- CD163阳性巨细胞通过骨质素 (OPN) 分泌促进RPF的进展.
- CD163巨-OPN相互作用代表了RPF的一个有前途的治疗标.
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