相关实验视频

Updated: Sep 19, 2025

The Use of Flow Cytometry to Assess the State of Chromatin in T Cells

The Use of Flow Cytometry to Assess the State of Chromatin in T Cells

Published on: December 17, 2015

细胞质组素是 CD4+ T 细胞的前炎性标记物

Bohan Xu^1,2, Guanglong Liu^1,2, Yifang Peng^1,2

¹Department of Pathology, Nanfang Hospital, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.

|June 4, 2025

概括

细胞质组素在 CD4+ T 效应细胞中升高,并在 T 细胞激活时增加. 这些组织蛋白水平可以作为炎症性CD4+T细胞和自身免疫性疾病 (如系统性红斑狼 (SLE)) 的生物标志物.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

众所周知,有核外基因组,但它们在淋巴细胞中的功能,特别是细胞质基因组,尚不清楚.
了解细胞质组素的作用对于淋巴细胞功能和疾病研究至关重要.

研究的目的:

为了研究细胞质组组织素在CD4+T细胞中的存在和作用.
确定细胞质组素水平是否与T细胞激活,分化和炎症状态相关.
探索细胞质组质素作为自身免疫性疾病的生物标志物的潜力.

主要方法:

视觉和定量分析CD4+T细胞中的细胞质组组素.
在T细胞受体 (TCR) 激活后和Th1/Th17分化过程中评估基因素水平.
研究双链RNA (dsRNA) 对Th1和Th17细胞中的细胞质组素的影响.
从系统性红斑狼 (SLE) 患者的CD4+T细胞中分析细胞质组素水平.

主要成果:

效应体 CD4+ T 细胞表现出比原始 T 细胞更高的细胞质组素水平.
TCR激活显著增加细胞质组组织素,在Th1和Th17分化过程中进一步升高.
双链RNA (dsRNA) 特别增强Th17细胞中的细胞质组素.
细胞质H2B阳性Th17细胞显示炎症性细胞因子表达的增加.
在SLE患者的CD4+T细胞中观察到细胞质组质组激素水平升高.

结论:

细胞质组素在激活和分化CD4+T细胞中升高,特别是在促炎子集中.
细胞质组素,特别是H2B,与Th17细胞中的炎症分子表达有关.
从SLE患者的CD4+T细胞中增加的细胞质组组织素表明它们在自身免疫病原发生中的作用.
细胞质组蛋白质代表了 CD4+ T 细胞和自身免疫性疾病的潜在生物标志物.

关键词:

CD4+ T 细胞是 CD4+ T 细胞.细胞因子细胞因子细胞质基因组素的细胞质基因组素这是一种炎症炎症炎症炎症.系统性红血性狼 (Systemic Lupus Erythematosus) 是一种全身性狼.

更多相关视频

Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers

Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers

Published on: March 6, 2009

Chromatin Immunoprecipitation ChIP to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells

Chromatin Immunoprecipitation ChIP to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells

Published on: July 29, 2010

相关概念视频

T Cell Activation and Clonal Selection

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...