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医院水槽生物膜的细菌再殖民

H G Healy¹, E Pawluk², L Dieter³

¹Department of Chemical and Environmental Engineering, Yale University, New Haven, CT, USA; Department of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA, USA; Yale Institute for Biospheric Studies, Yale University, New Haven, CT, USA.

The Journal of hospital infection

|June 4, 2025

概括

医院的水槽排放出导致医疗相关感染 (HAI) 的病原体. 常规清洁限制,但不排除, 24 小时内从下水道和水中的细菌再殖民.

科学领域:

环境微生物学环境微生物学
感染控制的方法是感染控制.
医院卫生医院卫生

背景情况:

医院的水槽排水是病原体的重要储库,有助于医疗相关感染 (HAI).
排水中的细菌可以通过水槽使用期间的滴滴分散传播到表面和患者.
有效地清洁水槽表面对于限制病原体传播至关重要.

研究的目的:

描述医院水槽组件中的微生物社区组成和丰富性.
评估清洗后水槽表面细菌再生的速度和主要来源.

主要方法:

从251个医院水槽收集了排水管,盖子,盆,饮用水和p-trap液体的样本.
使用培养计数,数字滴滴PCR,MALDI-ToF和16S rRNA基因测序来评估细菌的丰度和分类.
在表面清洁和消毒之前和之后分析了微生物群落.

主要成果:

排水生物膜显示了高的细菌丰富度 (中位数为1.80 × 10^8 16S rRNA基因拷贝/cm^2).
洗手盆表面的细菌水平与排水生物膜丰富度相关;走廊洗手盆的数量比患者房间洗手盆的数量更高.
清洗后,细菌在24小时内恢复到清洗前水平的80%以上,排水管和自来水是重定居的重要来源.

结论:

医院水槽表面在清洗后迅速重新定居,突出了常规消毒协议的局限性.
了解排水和水的贡献是改善感染控制策略的关键.
需要进一步的研究,以开发更有效的干预措施来对抗水槽相关的HAI.

关键词:

16S rRNA基因安普利康序列的测序哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈消毒消毒消毒消毒排水生物薄膜可以排水.饮用水饮用水饮用水预防感染预防感染微生物群落中的微生物群落.洗手盆的微生物群

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