在Bacillus subtilis中对蛋白质胺酶生产的益酶激活和代谢工程的调节
Maofang Teng1, Juan Zhang2, Jingwen Zhou2
1Science Center for Future Foods, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu, 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education and School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu, 214122, China.
Metabolic engineering
|June 4, 2025
概括
这项研究开发了一种新的Bacillus subtilis系统,用于高效的蛋白质胺酶 (PG) 生产. 优化的系统实现了成熟PG (mPG) 的高产量,克服了以前的生产挑战.
科学领域:
- 生物技术是生物技术.
- 酶学 是一种酶学.
- 微生物工程 微生物工程
背景情况:
- 蛋白质胺酶 (PG) 增强了植物蛋白的功能,但面临着低产量和基因操纵的挑战.
- 现有的PG生产方法效率低下,阻碍了工业应用.
研究的目的:
- 开发一种新的,自我激活的表达系统,用于Bacillus subtilis中的蛋白质胺酶 (PG).
- 克服天然PG生产的局限性,包括低产量和遗传复杂性.
主要方法:
- 工程 Bacillus subtilis 菌株用于识别亲PG (PPG) 激活蛋白酶.
- 优化基因表达组件 (促进剂,RBS) 用于PPG和蛋白酶共表达.
- 在麦芽糖代谢途径中敲除关键基因,以增强PG生产.
主要成果:
- 在B. subtilis.中确定了PPG激活蛋白酶和优化协同表达系统.
- 开发了一种工程菌株 (W8ΔM2-AE-Pmal380),显著提高了PG生产能力.
- 在64小时内在5L生物反应器中达到53.0U/mL的成熟PG (mPG) 产量.
结论:
- 使用 Bacillus subtilis.建立了一个有效的平台,用于工业蛋白质氨酶 (PG) 生产.
- 开发的系统为表达和激活其他益酶提供了有价值的参考.
- 成功解决了PG生产中低酶率和基因操纵的挑战.
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