PpMYC2和PpJAM2/3对抗性调节素合成,以应对桃子果的疾病
Qian Li1, Yi Chen1, Yingying Wei1
1State Key Laboratory for Quality and Safety of Agro-products, Zhejiang Key Laboratory of Intelligent Food Logistic and Processing, Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, College of Food Science and Engineering, Ningbo University, Ningbo, China.
Plant biotechnology journal
|June 4, 2025
概括
桃子通过积聚红素来增强疾病抵抗力. 上调的PpMYC2激活了红素基因,而下调的PpJAM2/3则减少了抑制,共同增强了水果对Monilinia fructicola的防御能力.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 转录因子MYC2和JAM是植物抗病的关键.
- 它们在非模型植物和收获后水果中的作用尚不清楚.
研究的目的:
- 调查PpMYC2和PpJAM2/3在桃花对Monilinia fructicola的耐药性中的作用.
- 阐明在收获后的桃子水果中潜在的疾病反应的分子机制.
主要方法:
- 基因表达分析 (RT-qPCR).
- 通过DNA亲和力净化和RNA测序 (DAP-seq).
- 双露西法酶记者测定,短暂的过度表达/沉默,电泳性移动性转移测定 (EMSA) 和酵母单杂交测定.
主要成果:
- 在M. fructicola感染后,PpMYC2的表达增加,而PpJAM2/3则下降.
- PpMYC2正调节了电阻;PpJAM2/3负调节了电阻.
- PpMYC2直接激活了五个素合成基因 (PpPAL1,PpC4H,Pp4CL1,PpCSE,PpCCoAOMT1),促进了素的积累.
- PpJAM2/3 抑制了特定的红素基因,并干扰了 PpMYC2 的结合.
结论:
- PpMYC2上调和PpJAM2/3下调协同促进素合成,以应对M. fructicola感染.
- 这种素的积累增强了收获后桃子果的抗病能力.
- 这项研究揭示了对收获后果病防御机制的新型分子洞察力.
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