针对ICU患者下呼吸道感染的广泛针对性下一代测序的性能:一项前性观察性研究
Chuanxi Chen1,2, Ruizhi Wang3,4, Bilin Wei1,2
1Department of Critical Care Medicine, The First Affiliated Hospital, Sun Yat-Sen University, No.58 Zhongshan Er Road, Guangzhou, Guangdong, China.
Critical care (London, England)
|June 4, 2025
概括
广谱向下一代测序 (bstNGS) 能够有效地检测下呼吸道感染 (LRTI) 的病原体,比传统方法更准确. 这种先进的技术显示出作为辅助诊断工具在重症监护室 (ICU) 的承诺.
科学领域:
- 医学诊断 医学诊断 医学诊断
- 微生物学 微生物学
- 基因组学就是基因组学.
背景情况:
- 目标下一代测序 (tNGS) 是一种先进的诊断技术,具有检测感染的潜力.
- tNGS的确切临床应用,特别是下呼吸道感染 (LRTI),需要进一步定义.
研究的目的:
- 评估广谱向下一代测序 (bstNGS) 的临床实用性和诊断性能,用于识别LRTIs患者的病原体.
主要方法:
- 一项前性观察性研究比较了bstNGS,下一代元基因组测序 (mNGS) 和传统培养方法,用于检测重症患者的支气管支气管洗液 (BALF) 中的病原体.
- 后勤回归分析了影响bstNGS检测的临床变量,并检查了bstNGS结果与临床结果之间的相关性.
主要成果:
- bstNGS与mNGS (96.33%) 和培养 (91.15%) 具有很高的一致性,在较低的负载下识别病原体.
- 与培养 (46.00%) 相比,bstNGS (87.33%) 和mNGS (82.00%) 的病原体检测率明显高 (p < 0.05).
- bstNGS比mNGS (86.00%) 和培养 (49.33%) (分别p < 0.05和p < 0.0001) 显示出更高的诊断准确性 (90.67%). 免疫性损害与较低的检测效率有关 (p=0.04).
结论:
- bstNGS是一种有效的方法来检测LRTIs中的病原体.
- 这项研究支持bstNGS作为重症监护室 (ICU) 设置中管理呼吸道感染的有价值的辅助诊断工具.
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