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时钟和与时钟相关的基因表达在大西洋鱼 (Salmo salar) 胚胎中对光有反应

Marie Bue¹, Øivind Andersen^2,3, Helge Tveiten¹

¹The Norwegian College of Fishery Science, UiT - the Arctic University of Norway, Tromsø, Norway.

Chronobiology international

|June 5, 2025

概括

鱼的早期发育受到光线的影响. 模拟的自然光周期 (LD) 增强了时钟和黑激素基因的表达,而不是连续的光或黑暗,这表明照明对发育有影响.

科学领域:

水产养殖是水产养殖的一种方式.
时间生物学时间生物学
分子生物学分子生物学

背景情况:

光和光周期是调节生物过程的关键环境线索.
水产养殖行业使用人工光来操纵鱼类的发育,但其对早期鱼本体发生的影响尚未完全理解.
了解光照对基因表达的影响对于优化鱼养殖实践至关重要.

研究的目的:

研究不同照明模式对早期鱼发育中的时钟和黑素相关基因表达的影响.
确定连续的黑暗,连续的光和模拟的自然光周期 (LD) 如何影响不同发育阶段的基因表达 (眼前,眼后,开始养前).

主要方法:

鱼蛋和幼虫被暴露在三个不同的光照模式下:持续的黑暗,持续的光和压缩的模拟自然光周期 (LD).
分析了八个关键时钟和黑素相关基因 (clock1a.2, arntl1a.2, per1b, per2a, cry3b, nr1d1a, aanat2b, mtnr1b) 的表达水平.
基因表达在一天周期内在三个关键发育时间点量化.

主要成果:

大多数时钟和黑激素基因在整个发育过程中表达的增加,其中一些 (clock1a.2,per2a) 可能是母体沉积的.
在三种光照模式中观察到基因表达的显著差异.
与连续光或暗相比,模拟的自然光周期 (LD) 模式导致时钟基因的节奏表达更加丰富和强烈. 值得注意的是,所有检查的基因在开始养阶段在LD下表现出节奏表达.

结论:

光线条件在早期鱼发育过程中显著影响昼夜钟和黑素相关基因的表达.
模拟的自然光周期 (LD) 似乎是促进发育至关重要的节律基因表达的最有效的照明模式.
这些发现强调了在水产养殖中仔细控制照明条件的重要性,以确保最佳的鱼生长,并可能避免长期的发展问题.

关键词:

循环节律是循环节律的节奏.一个钟表的钟表黑激素是什么意思黑激素是什么意思人类的本体生长过程 (ontogeny)摄影周期的摄影周期鱼鱼鱼鱼鱼

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