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Updated: Sep 19, 2025

Using High Content Imaging to Quantify Target Engagement in Adherent Cells
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Using High Content Imaging to Quantify Target Engagement in Adherent Cells

Published on: November 29, 2018

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针对性共价欧标签的抗体导向细胞内化使得在现场激酶标记和诱导联等离子体质谱 (ICP-MS) 量化能够实现

Ziyang Fang1, Xinyue Zhao1, Ruxue Hou1

  • 1Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

Analytical chemistry
|June 5, 2025
PubMed
概括

我们开发了一种新的基于抗体的方法,使用诱导合等离子体质谱法 (ICP-MS) 来标记和量化活细胞内的蛋白质. 这种方法改善了用于诊断和药物开发的细胞内蛋白质检测.

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Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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