在Allotetraploid Actinidia valvata中亚基因组占主导地位 调节RNA m6A 修改为水浸耐受性
Xiaoli Hu1,2, Changbin Xu1,2, Xiaolan Li1,2
1National Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou, Zhejiang, 311300, China.
概括
基维果亚基因组的优势增强了对浸水的耐受性. 这发生在主导亚基因组中的表观遗传调节和基因表达,这对植物适应至关重要.
科学领域:
- 植物基因组学 植物基因组学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 进化生物学是进化的生物学.
背景情况:
- 多化驱动了植物的进化和适应,特别是在果中.
- 多倍体中的亚基因组相互作用会影响诸如活力和应激弹性之类的特征.
- 这些相互作用背后的分子机制在很大程度上是未知的.
研究的目的:
- 为了研究四体基维果 (Actinidia valvata) 中亚基因组主导性和浸水耐受性的分子基础.
- 了解表观遗传修饰和基因表达如何促进多体植物的抗压能力.
主要方法:
- 高质量的Actinidia valvata的基因组测序.
- 对可转移元素,DNA甲基化和染色质可访问性的分析.
- RNA测序和评估RNAN6甲基氨酸 (m6A) 甲基化.
- 实验性抑制RNAm6在水浸压下在根中的甲基化.
主要成果:
- 阿克丁尼迪亚瓦尔瓦塔基因组是混合起源的,具有主导的亚基因组.
- 主导亚基因组显示了增强的基因表达,更少的可转移元素,更低的DNA甲基化和更高的染色质可访问性.
- 在多倍体化之前,子基因组的主导地位已经部分确立.
- 主导的亚基因组对浸水压力更有反应,从其原始基因遗传了耐受性.
- 抑制RNAm6的甲基化改善了水浸下的根部活动.
- 浸水应激调节了m6A的修饰,特别是在主导的亚基因组中.
结论:
- 在Actinidia valvata中亚基因组的主导地位是其特殊的浸水耐受性的关键因素.
- 多种调节机制,包括表观遗传修饰和偏向RNAm6A甲基化,支持亚基因组的主导地位.
- 这些发现提供了关于植物多化和适应的进化意义的见解.
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