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伦丁斯 (Lentinus edodes) 的结合机制与HP-β-CD与埃尔戈斯特醇结合:来自in silico模拟的见解

Xin Cai¹, Bo Liu², Lingmin He³

¹College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, China; School of Biological and Food Engineering, Anyang Institute of Technology, Anyang, Henan 455000, China.

|June 5, 2025

概括

这项研究表明,2-基-β-环极素 (HP-β-CD) 可以与厄戈斯特醇形成稳定的含有复合物. 这种复合物显著提高了埃尔戈斯特的可溶性,稳定性和生物活性,以更好地传递.

科学领域:

药理学药理学是指药理学的学科.
材料科学材料科学材料科学
生物化学生化学

背景情况:

埃尔戈斯特醇是像Lentinus edodes这样的真菌中的一个关键类固醇,由于溶解性和稳定性差,其应用有限.
提高埃尔戈斯特的物理化学性质对于其在各种领域的有效利用至关重要.

研究的目的:

为了研究埃尔戈斯特醇和2-propyl-β-cyclodextrin (HP-β-CD) 之间形成稳定的包容复合物 (IC).
评估这种IC对爱尔戈斯特的可溶性,稳定性,生物可访问性,抗氧化活性和降低胆固醇潜力的影响.

主要方法:

利用信号因子和Box-Behnken设计来优化IC形成.
采用分子对接,动力学模拟和多光谱分析来阐明包含机制.
评估了IC属性,包括封装效率,溶解度,应激条件下的稳定性,生物可访问性和抗氧化活性.

主要成果:

在优化条件下达到86.4%的最大封装效率 (1:10.5核心-外比,400rpm,32h).
埃尔戈斯特-HP-β-CD集成电路表现出对光和热的增强稳定性.
与自由厄戈斯特相比,显著提高了生物可访问性,抗氧化活性和降低胆固醇.
证实了ERGOSTEROL在IC内从晶体变为无形的转化,使其水溶性增加到66.7μg/mL.

结论:

由埃尔戈斯特和HP-β-CD形成的包容复合体提供了一种可行的策略,以克服埃尔戈斯特的可溶性和稳定性限制.
这种方法显著提高了ergosterol的功能性质,为改善治疗和营养药物应用铺平了道路.

关键词:

生物可访问性生物可访问性这是一个计算机模拟.交付系统的交付系统.埃尔戈斯特醇是一种包容性复杂的包容性复杂的互动互动互动互动

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