一份简短的指南,分析基于TurboID的植物近距离标记质谱
1Department of Life Science, Hanyang University, Seoul 04763, Republic of Korea; Hanyang Institute of Bioscience and Biotechnology, Hanyang University, Seoul 04763, Republic of Korea.
Molecules and cells
|June 5, 2025
概括
近距离标记质谱法 (PL-MS) 是研究蛋白质相互作用的关键方法. 本研究详细介绍了基于TurboID的植物细胞的PL策略,涵盖了从植物准备到数据分析的实验步骤.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 植物科学 植物科学
背景情况:
- 与质谱学 (PL-MS) 相结合的近距离标记对于绘制蛋白质互动体的地图至关重要.
- 了解细胞内蛋白质-蛋白质相互作用对于破译细胞功能至关重要.
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