Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

10.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.4K
Ribosome Profiling02:24

Ribosome Profiling

3.7K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

HeartBioPortal 3.0: an integrated cardiovascular genomics knowledge environment for molecular, clinical and population-scale interpretation.

medRxiv : the preprint server for health sciences·2026
Same author

Current trends and challenges in deciphering single molecule resolution maps of single cell transcriptomes.

Briefings in bioinformatics·2026
Same author

Regulation Ratio: A Singular Multi-Omic Measurement of Gene Regulatory Mechanisms.

Computational and structural biotechnology journal·2026
Same author

Single-Cell Transcriptomic Analysis of Salivary Epithelial Cells Reveals Large-Scale Dysregulation in Bitter Taste Dysfunction.

International journal of molecular sciences·2026
Same author

Genotype-Based Severity Scoring System in Wolfram Syndrome: Correlation with Onset of Cardinal Symptoms and <i>WFS1</i> Gene Variant Types.

medRxiv : the preprint server for health sciences·2026
Same author

BigEye: a clinically interpretable deep learning framework for diabetic retinopathy detection and stage prediction.

Scientific reports·2026

相关实验视频

Updated: Sep 19, 2025

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

13.7K

从单个分子转录组中研究RNA动态.

Sahiti Somalraju1, Doaa Hassan Salem2, Sarath Chandra Janga3

  • 1Department of Biomedical Engineering and Informatics, Luddy School of Informatics, Computing and Engineering, Indiana University Indianapolis (IUI), Indianapolis, IN, USA.

Trends in genetics : TIG
|June 5, 2025
PubMed
概括

像PacBio和ONT这样的长读测序技术揭示了RNA动态,包括拼接和修改. 这些方法为研究高分辨率的基因调节和细胞过程提供了新的途径.

关键词:
在RNA生物信息学方面.有关RNA的RNA异形.基因组RNA的修改 基因组RNA的改变多种 (A) 尾部单个分子测序的测序.翻译学 翻译学 翻译学 翻译学

更多相关视频

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

912
Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture
11:00

Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture

Published on: August 8, 2013

27.4K

相关实验视频

Last Updated: Sep 19, 2025

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

13.7K
Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

912
Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture
11:00

Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture

Published on: August 8, 2013

27.4K

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 了解RNA动态是细胞功能和疾病的关键.
  • 替代拼接,RNA稳定性和修改是关键的RNA过程.
  • 治疗标的识别依赖于理解RNA调节.

研究的目的:

  • 审查使用长读测序分析RNA动态的协议和方法.
  • 要突出异构体检测的进步,多个A尾的量化,和RNA修饰映射.
  • 讨论这些技术在改变RNA研究中的潜力.

主要方法:

  • 专注于长期阅读的测序技术,特别是太平洋生物科学 (PacBio) 和牛津纳米孔技术 (ONT).
  • 检查分析单分子RNA动态的协议.
  • 与软件系统集成高吞吐量,全转录组数据.

主要成果:

  • 长读测序提供了RNA动态的单分子和单核酸解析.
  • 已确定的方法用于异形检测,多A尾长量化和RNA修饰映射.
  • 开发用于数据分析的综合软件系统.

结论:

  • 长时间读取的测序技术正在彻底改变RNA动态的研究.
  • 这些进展为基因调节和细胞过程提供了更深入的见解.
  • 未来的工作流程将利用高通量数据进行全面的RNA分析.