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响应pH的益生菌-脂质体混合系统通过抗炎和微生物组调节的双重机制协同治疗辐射诱导的损伤
Heshuo Fan1, Chen Li2, Zhehao Zhang1
1Department of Pathology at the First Affiliated Hospital, State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection & School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Medical College, Soochow University, Suzhou, 215123, China.
一种新的益生菌-脂质体系统通过减少炎症和恢复肠道细菌,有效地治疗辐射诱导的肠道损伤. 这种双作用疗法对接受放射治疗的患者来说是有前途的.
科学领域:
- 生物医学工程 生物医学工程
- 微生物学 微生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 放射治疗通常会导致肠道损伤,导致炎症和失生症.
- 目前的治疗方法在疗效和副作用方面存在局限性.
研究的目的:
- 开发一种pH响应的益生菌-脂质体混合系统 (EcN-PIN-L) 用于治疗辐射诱导的肠损伤.
- 在小鼠模型中评估 EcNPIN-L 的治疗疗效.
主要方法:
- 结合大肠杆菌尼斯尔1917 (EcN) 与皮诺布林 (PIN) 装载的脂质体,通过生物-斯特雷普塔维丁结合.
- 将混合系统封装在Eudragit L100-55中,用于口服.
- 在被辐射的小鼠中评估肠道殖民,细胞因子水平,屏障功能,亡和微生物群组成.
主要成果:
- EcNPIN-L证明了有效的肠道殖民和pH响应的释放.
- 该系统显著降低了促炎性细胞因子 (TNF-α,IL-17A) 和上皮细胞亡.
- 观察到微生物群恒温的改善,有益细菌增加,有害物种减少.
- EcNPIN-L与单个成分相比显示出更高的疗效.
结论:
- EcNPIN-L是一种有前途的双机制疗法,用于辐射诱导的肠损伤.
- 该系统提供有针对性的输送,抗炎作用和微生物组调节.
- 这种方法解决了炎症损伤和微生物失生症,具有良好的生物相容性.
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