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光亲和SAM类似物用于识别SAM结合蛋白

Xiangyu Wu¹, Min Dong^1,2,3

¹State Key Laboratory of Synthetic Biology, Frontiers Science Center for Synthetic Biology, School of Chemical Engineering and Technology, Tianjin University Tianjin 300072 China mindong@tju.edu.cn.

Chemical science

|June 6, 2025

概括

研究人员开发了新的S-Adenosylmethionine (SAM) 光亲和探针,以识别未知的SAM结合蛋白. 这些探测器成功标记了已知的蛋白质,并揭示了具有分裂活动的新SAM结合酶.

科学领域:

生物化学生化学
分子生物学分子生物学
蛋白质组学是指蛋白质组学.

背景情况:

S-Adenosylmethionine (SAM) 是一个关键的生物基质,参与许多生化反应.
识别SAM结合蛋白对于全面了解SAM的生物作用至关重要.
之前的方法主要确定了依赖SAM的甲基转移酶,可能缺少其他与SAM相互作用的蛋白质.

研究的目的:

开发和验证用于标记和丰富SAM结合蛋白的新型SAM光亲和探针.
识别以前未知的SAM结合蛋白并描述它们的功能.
扩大用于研究SAM-蛋白相互作用的工具包.

主要方法:

开发和验证三种不同的SAM光亲和探测器.
使用探针标记和丰富来自细胞溶解物的SAM结合蛋白.
丰富蛋白质的蛋白质组分析以确定SAM结合剂.
生物化学测试以确定已识别的蛋白质的SAM裂变活动.

主要成果:

开发的SAM光亲和探测器有效地标记了已知的SAM结合蛋白,例如Dph2.
从*Burkholderia gladioli*和*Saccharomyces cerevisiae*的细胞溶解物中成功丰富了SAM结合蛋白.
五种新的SAM结合剂得到了验证,其中三种表现出SAM裂解活动,包括ArsL,AcnA和EDD84_07545.

结论:

基于SAM的新型光 afinity探针是识别SAM结合蛋白的有效工具.
这些探测器有助于发现参与SAM代谢和功能中的蛋白质.
这些发现扩大了已知的SAM相互作用蛋白和它们的酶活性.

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