皮基-1是一种II类的酸丁醇3-激酶,在内细胞贩运中起作用
bioRxiv : the preprint server for biology
|June 6, 2025
概括
我们确定了PIKI-1,一种酸氨基 3-激酶,对于在C. elegans中产生PI(3,4) P2至关重要. 它的减少抑制了由NEKL-2耗尽引起的膜流通和变缺陷.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 膜运输对细胞功能至关重要,由蛋白质,脂质和细胞骨调节.
- 与NIMA相关的激酶NEKL-2和NEKL-3是C. elegans中膜贩运和变的关键调节者.
研究的目的:
- 为了识别nekl相关的变缺陷的遗传抑制剂.
- 为了调查酸丁3-激酶 (PI3Ks) 在C. elegans膜贩运中的作用.
主要方法:
- 基因查以隔离 piki-1.1 中的功能减弱突变.
- 使用基因编码的脂质传感器测量PI(3) P和PI(3,4) P2水平.
- 通过显微镜观察早期内体形态和蛋白质组成.
主要成果:
- 作为II类PI3K的一种,PIKI-1是C. elegans表皮中PI(3,4) P2的主要生产者.
- PIKI-1和PI{3,4) P2定位在早期的内体,而PIKI-1的减少会影响它们的形态和蛋白质含量.
- 减少PIKI-1功能抑制了nekl-2-相关的变缺陷和内体异常.
结论:
- 通过PIKI-1-介导的PI(3,4) P2的产生对于正常的早期内体功能和C. elegans的变形是必不可少的.
- 调节PI{3,4) P2水平及其结合伙伴,如HIPR-1,为NEKL相关疾病提供了潜在的治疗策略.
更多相关视频
07:26Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
Published on: October 15, 2016
9.5K
06:47High-throughput Measurement of Dictyostelium discoideum Macropinocytosis by Flow Cytometry
Published on: September 10, 2018
7.7K
相关概念视频
Phosphoinositides and PIPs
8.5K
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
8.5K
IP3/DAG Signaling Pathway
12.0K
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
12.0K
PI3K/mTOR/AKT Signaling Pathway
3.5K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.5K
The JAK-STAT Signaling Pathway
8.7K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
8.7K
Amplifying Signals via Second Messengers
6.8K
Many receptor binding ligands are hydrophilic; they do not cross the cell membrane but bind to cell-surface receptors. Thus, their message must be relayed by second messengers present in the cell cytoplasm. There are several second messenger pathways, each with its own way of relaying information. For example, the G protein-coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol pathway is active when the receptor induces...
6.8K
GPI Anchoring of Proteins in the ER Membrane
4.0K
GPI-anchoring is a post-translational, reversible protein modification that is ubiquitous in eukaryotes. Such proteins are primarily present on the exoplasmic leaflet of the plasma membrane.
GPI-anchor structure
A sequence of 11 enzymatic reactions results in the synthesis of the complete GPI anchor consisting of a hydrophobic and a hydrophilic portion. The hydrophobic portion comprises phosphatidylinositol, while the hydrophilic part comprises polar groups like phosphoethanolamine,...
GPI-anchor structure
A sequence of 11 enzymatic reactions results in the synthesis of the complete GPI anchor consisting of a hydrophobic and a hydrophilic portion. The hydrophobic portion comprises phosphatidylinositol, while the hydrophilic part comprises polar groups like phosphoethanolamine,...
4.0K
