相关实验视频

Updated: Sep 19, 2025

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Published on: July 2, 2016

通过小RNA-seq进行Culex tarsalis的病毒样本分析:对低于最佳样本的挑战

Jaime Manzano-Alvarez^1,2, Sultan Asad^1,2, Duverney Chaverra-Rodriguez^1,2,3

¹Department of Entomology, The Pennsylvania State University, University Park, PA, 16802, USA.

bioRxiv : the preprint server for biology

|June 6, 2025

概括

小RNA测序可靠地确定了Culex tarsalis蚊子中的七种病毒,即使是在具有挑战性的现场条件下收集的样本中也是如此. 这种方法有助于了解蚊子病毒和昆虫特有的病毒.

科学领域:

病毒学病毒学
昆虫学昆虫学是一门学科.
分子生物学分子生物学

背景情况:

蚊子传播的病毒如西尼罗河病毒 (WNV) 构成公共卫生风险.
在北美,Culex tarsalis蚊子是显著的WNV载体.
昆虫特异性病毒 (ISV) 可以影响蚊子中的WNV复制,需要对病毒体进行表征.

研究的目的:

研究美国中西部的Culex tarsalis种群的病毒组.
评估小RNA测序 (sRNA-seq) 在低于最佳现场条件下的病毒组分析的实用性.
描述与已识别的病毒相关的病毒衍生小RNA (vsRNA) 资料.

主要方法:

在Culex tarsalis蚊子样本上利用sRNA测序 (sRNA-seq) 来自5个中西部州的17个地点.
在COVID-19大流行期间,在具有挑战性的现场条件下收集的样本进行分析.
鉴定和表征了七种相关病毒及其siRNA和piRNA配置文件.

主要成果:

在Culex tarsalis种群中成功识别了七种不同的病毒.
描述了每个已识别的病毒的特定小RNA (siRNA和piRNA) 配置文件.
证明了sRNA-seq在病毒组分析中的强度和可靠性,尽管样本完整性存在挑战.

结论:

sRNA-seq是一个有价值的工具来表征蚊子病毒组,即使样本质量受到损害.
这项研究扩大了对Culex tarsalis.ISVs的知识.
这些发现支持在真实世界监测场景中使用sRNA-seq进行载体传播疾病研究.

关键词:

在RNA-Seqq.病毒组病毒组病毒组蚊子蚊子蚊子这就是为什么piRNARNA.它们是sRNARNA.siRNA 是一个RNA.

更多相关视频

Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq

Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq

Published on: April 7, 2013

Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes

Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes

Published on: August 31, 2022

相关概念视频

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

Viruses with RNA Genomes

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...