相关实验视频

Updated: Sep 19, 2025

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

瘤性 lncRNA 转基因转录调节了原始染色体位置上的表观遗传记忆

Sweta Sikder¹, Songjoon Baek¹, Yamini Dalal¹

¹Chromosome Structure and Epigenetic Mechanisms Unit, Laboratory of Receptor Biology and Gene Expression, Center for Cancer Research, NCI, NIH, Bethesda, MD 20892, USA.

bioRxiv : the preprint server for biology

|June 6, 2025

概括

细胞可以通过沉默转基因来抑制异位中心体的形成. 这种表观遗传修复阻止了新中心体,除非选择有利于它们的形成,保持基因组的完整性.

科学领域:

遗传学遗传学是一个
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学

背景情况:

基因组完整性对于生物体的发育和功能至关重要.
在非中心体位的新中心体形成是基因组稳定性的一个有趣方面.
中间体蛋白A (CENP-A) 对于中间体的认同至关重要,但在癌症中会发生它的异位错位.

研究的目的:

研究实验诱导的宫外CENP-A位点的寿命和稳定性.
了解控制子宫外中心体形成的维持或丧失的表观遗传机制.
探索细胞防故障机制,以防止在非正规地点形成新中心体.

主要方法:

通过使用致癌性lncRNA (PCAT2) 设计了一个稳定的异胎CENP-A位点,以招募CENP-A到非中心体转录位点.
携带稳定转基因的追踪细胞,随着时间的推移监测诱导的宫外CENP-A位点.
分析了表观遗传沉默机制及其对转基因表达和宫外CENP-A水平的影响.

主要成果:

宫外CENP-A部位的诱导表观遗传记忆最终被丢失了.
竞争的表观遗传沉默机制抑制了负责异胎CENP-A招募的转基因.
在工程部位的子宫外CENP-A水平自然逆转至基线水平.

结论:

细胞具有固有的安全机制,可以防止新中心体在子宫外部形成.
抑制转录是抵消子宫外CENP-A沉积的关键机制.
在子宫外部部位形成的新中心体可能只会持续,如果受到涉及多个因素的强有力的选择性压力.

关键词:

这就是CENP-A.染色体的不稳定性表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.异性染色是一种异性染色.这是一个LncRNARNA.

更多相关视频

CRISPR-Mediated Reorganization of Chromatin Loop Structure

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Published on: September 14, 2018

Author Spotlight: Enhancements in Gene Expression Regulation Research

Author Spotlight: Enhancements in Gene Expression Regulation Research

Published on: September 15, 2023

相关概念视频

Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

Inheritance of Chromatin Structures

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...

Chromatin Position Affects Gene Expression

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...

Cancer-Critical Genes I: Proto-oncogenes

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...

Methods of Nuclear Reprogramming

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...