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在Corynebacterium pseudotuberculosis biovar ovis的粘附和内化后,MDBK细胞系中的细胞病变影响

A A Gallardo¹, V Azevedo², R Malena³

¹Cátedra de Microbiología Clínica, Departamento de Bioquímica, Facultad de Ciencias Naturales y Ciencias de La Salud, , Universidad Nacional de La Patagonia San Juan Bosco (UNPSJB), Comodoro Rivadavia, Chubut, Argentina. adryarg@yahoo.com.ar.

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]

|June 6, 2025

概括

科里尼细菌伪结核病,是畜牧动物中病变性淋巴腺炎的原因,可以粘附并侵入宿主细胞. 这种细菌保持活力长达72小时,导致不同程度的细胞损伤.

科学领域:

兽医微生物学兽医微生物学
细菌病原体的产生
细胞生物学细胞生物学

背景情况:

病状淋巴腺炎是一种慢性,传染性疾病,主要影响绵羊和山羊.
科里尼细菌伪结核病的毒性与脂酶D和酸有关,有助于的形成和传播.
了解细菌与宿主细胞的相互作用对于疾病管理至关重要.

研究的目的:

为了评估Corynebacterium pseudotuberculosis biovar ovis在非食细胞细胞系中的粘附和内化.
评估C. pseudotuberculosis biovar ovis在Madin-Darb牛 (MDBK) 细胞中诱导的细胞病变影响.

主要方法:

使用了6种C. pseudotuberculosis biovar ovis的分离物.
在MDBK细胞中评估了粘附和内化.
用扫描电子显微镜和光显微镜下Giemsa染色来分析细菌相互作用和细胞病变效应在感染后6,24和72小时.

主要成果:

C. pseudotuberculosis biovar ovis表现出粘附并入侵MDBK细胞的能力.
细菌在细胞培养物中保持活力长达72小时.
观察到不同程度的细胞病变效应,包括细胞质真空形成,核/细胞质比变化和细胞溶解.

结论:

科里尼细菌 (Corynebacterium pseudotuberculosis biovar ovis) 能够成功地附着并入侵非吞细胞宿主细胞.
病原体在宿主细胞内表现出长时间的活力,有助于疾病的进展.
这项研究强调了C. pseudotuberculosis biovar ovis在相关细胞模型中的细胞病变潜力.

关键词:

C. 伪结核病伪结核病有病变性淋巴腺炎的病例性淋巴腺炎细胞病变影响细胞病变影响非食细胞细胞系的细胞系.

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