下调THBS4通过TGF-β1/Smad信号级联改变了孕前的热囊细胞功能
Haoran Shi1, Yan Mao1,2, Jianjian Cui1
1Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
血栓松丁-4 (THBS4) 在孕前症的胎盘组织中降低. 减少THBS4通过转化生长因子 (TGF) -β1通路损害了热囊细胞功能,影响了胎盘血管发育.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
- 病理学 病理学 病理学
背景情况:
- 孕前 (PE) 是一种特定于怀孕的高血压疾病.
- 损伤的 trofhoblast 细胞入侵和子宫动脉重塑有助于 PE 病原体.
- 了解 trofhoblast 功能的分子调节者对于 PE 研究至关重要.
研究的目的:
- 确定影响热囊细胞入侵和迁移的关键分子.
- 为了研究血栓素-4 (THBS4) 在孕前症中的作用.
主要方法:
- 从PE和正常怀孕的胎盘组织中THBS4表达的定量分析.
- 使用HTR-8/SVneo热原体细胞进行体外研究以评估THBS4功能.
- 操纵THBS4表达和TGF-β1信号通路的激活.
主要成果:
- 与正常怀孕相比,孕前患者的胎盘组织中THBS4表达显着较低.
- 在 trofhoblast 细胞中 THBS4 的下调抑制了增殖,迁移和入侵.
- THBS4的淘汰抑制了转化生长因子 (TGF) -β1信号通路.
- 在治疗TGF-β1主动剂 (酸盐) 后观察到THBS4淘汰效应的逆转.
结论:
- THBS4在 trofhoblast 细胞的生物功能中起着调节作用.
- TGF-β1信号通路与THBS4介导的 trofhoblast 行为的调节有关.
- THBS4可能是胎盘血管发育的重要因素,可能是孕前的病因.
更多相关视频
11:44A Primary Human Trophoblast Model to Study the Effect of Inflammation Associated with Maternal Obesity on Regulation of Autophagy in the Placenta
Published on: September 27, 2017
12:02Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption
Published on: July 30, 2016
相关概念视频
TGF - β Signaling Pathway
Regulation of Angiogenesis and Blood Supply
Teratogenicity
Hormonal Regulation
The JAK-STAT Signaling Pathway
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
